HI-6 TREATMENT DOES NOT REACTIVATE SARIN INHIBITED ACETYLCHOLINESTERASE ACTIVITY IN DOG BRAIN WHEN ADMINISTERED IN HUMAN THERAPEUTICAL DOSE 30 MINUTES AFTER THE POISONING
Filip Caisberger, Ladislav Novotny, Petr Hajek, Jan Misik, Jiri Kassa, Jaroslav Pejchal
2016, 2 - 7
Hana Tomaskova, Jitka Kuhnova, Kamil Kuca
2016, 8 - 12
International terrorism is rightly regarded as one of the greatest threats to world security and stability. Terrorist attacks that have occurred in recent years around the world have highlighted the need for a comprehensive approach. Formulation of the problem is a prerequisite for successful work, but the main problem is that there is no single definition of terrorism. View on possible definitions and important concepts are mentioned in this article.
Vladimir Pavlik, Jana Fajfrova, Jan M. Horacek, Vaclav Safka, Pavla Krutisova, Miroslav Urban, Peter Urban, Jana Gazdackova, Petr Navratil
2016, 13 - 18
HUMULUS LUPULUS L. (HOPS) – A VALUABLE SOURCE OF COMPOUNDS WITH BIOACTIVE EFFECTS FOR FUTURE THERAPIES
Jana Olsovska, Vanda Bostikova , Martin Dusek, Vladimira Jandovska, Katerina Bogdanova, Pavel Cermak, Pavel Bostik, Alexandr Mikyska, Milan Kolar
2016, 19 - 30
Radek Pudil, Martina Vasatova, Alena Myslivcova Fucikova, Helena Rehulkova, Pavel Rehulka, Vladimir Palicka, Jiri Stulik
2016, 31 - 38
The natural history of hypertrophic cardiomyopathy (HCM) varies from an asymptomatic and benign clinical course to sudden premature death. Therefore, the new markers are searched with the aim to detect risk patients and improve their prognosis. The aim of this study was to test a cardiac multimarker testing strategy in detection of initial structural changes in patients with nonobstructive hypertrophic cardiomyopathy (HCM). In the group of 47 patients with nonobstructive HCM (58.4 ± 12.4 years, 12 emales) the mean left ventricle mass was 344.8 ± 129.9 g, the mean left ventricle mass index was 171.4 ± 60.2 g.m-2. We observed increased concentration of cardiac markers in peripheral blood: high sensitivity troponin T (hsTnT): median: 9 ng/L (IQR: 5 - 16 ng/L), vs. controls: 7 (5 - 9) ng/L, p 0.03; creatine kinase MB isoenzyme (CK MB): 2 (1.4 - 2.7) μg/L vs. 1.6 (1.1 - 2.2) μg/L, p 0.04; myoglobin 46.4 (33.3 - 65.2) μg/L vs. 35.6 (22.8 - 43.7) μg/L, p 0.001; heart type of fatty acid binding protein (hFABP): 1.8 (1.4 - 3.3) μg/L vs. 1.6 (1.3 - 2.1) μg/L, p 0.05; glycogen phosphorylase BB (GPBB): 3.9 (2.5 - 6.3) μg/L vs. 2.3 (1.9 - 4.2) μg/L, p 0.001. The analysis of the associations of left ventricle mass index and cardiac markers revealed its significant association with hFABP (r = 0.41, 95% CI: 0.07-0.66, p 0.01), CKMB (r = 0.33, 95% CI: 0.11-0.59, p 0.05), and with hsTnT (r = 0.39, 95%CI: 0.12 - 0.62, p 0.008). This study indicates potential clinical use of the multimarker testing in diagnosis and screening of the hypertrophic cardiomyopathy.
2016, 39 - 43
The Syrian civil war is a shameful matter for the contemporary world. It is full of suffering for the civilian population and is one of the causes of the current huge migration of Syrian people in Europe. Suffering of people affected by the war is still escalating due to use of banned chemical weapons. The entire civilized world is appalled by the atrocities of the Syrian civil war.
2016, 44 - 46
Geneva, Switzerland, celebrated a most remarkable 90th birthday jubilee. A highly respected Canadian surgeon who enjoys excellent mental and physical condition, and continues to perform the scientific and organizational work for which he has long been recognized throughout the world. A pioneer of medical emergency systems, Professor Gunn has contributed greatly to the emergence and evolution of Disaster Medicine, Humanitarian Medicine, and the expansion of surgical fields in developing and “third world” countries. He worked closely with officials for many countries and has continued this collaboration with Czech healthcare and medical educational institutions; most notably with Charles University in Prague and ...
2016, 47 - 47
Jiri Patocka, Kamil Kuca
2016, 50 - 55
Sternutators are substances that irritate the nasal and respiratory passages and cause coughing, sneezing, lacrimation, and sometimes vomiting. They are organoarsenic compounds and some of them were used as warfare toxic gases in World War I, namely diphenylaminochloroarsine, diphenylchloroarsine, diphenylcyanarsine, and phenyldichloroarsine. The effect of these irritants is in principle non-lethal.
CURRENT ADJUSTMENTS IN TEACHING AND TRAINING STUDENTS AT THE FACULTY OF MILITARY HEALTH SCIENCES, UNIVERSITY OF DEFENCE
Milan Ruzicka, Vojtech Humlicek, Michal Potac, Jaroslav Zdara, Petra Vrtiskova
2016, 56 - 62
The University of Defence (UD) provides comprehensive education and training of students to get knowledge and skills which enable graduates to manage activities in the area of management and leadership. Formerly, the UD students received military and leadership skills especially within the military training outside the UD in basic training, in the Career course or the Specialized officer course at the Training Command - Military Academy (TC-MA) in Vyškov. Currently, the UD has included the Officer course into its own teaching curriculum, and thus bears a full responsibility for comprehensive preparedness of the UD graduates for their assignment to systemized positions in the Army of the Czech Republic (ACR).
Shreesh Ojha, Syed M. Nurulain, S. Dhanasekaran, Mohammad Shafiullah, Abdu Adem, Charu Sharma, Kamil Kuca, Huba Kalasz
2016, 63 - 68
Martina Vrbova, Eva Dastychova, Tomas Rousar
2016, 69 - 74
The kidneys are one of the organ that can be commonly damaged by a number of toxic compounds (heavy metals, xenobiotics, drugs, etc.). To characterize the mechanism of toxicity, a variety of methods have been developed. The in vitro methods belong among the mostly used. Especially, the use of cell lines seems to be the leading approach to test and to characterize the toxicity mechanisms. At present, several cell lines of animal (from rat, dog, pig) or human origin are available. A detailed evaluation must go before any selection of a suitable cell line for experiments. Therefore, the aim of this review was to describe and to evaluate the mostly used renal cell lines.
2016, 75 - 79
New and modern techniques of drug design are extensively used in parallel or instead of the classic ones. Applicability of virtual screening (VS) is growing with the computational performance. This article includes list and short description of most frequent used methods in VS. These methods are divided into two groups – ligand-based VS and structure-based VS. Ligand based methods include chemical similarity, pharmacophore and quantitative structure-activity relationship. Molecular docking and scoring are methods of the structure-based VS.
2016, 80 - 88
Beauveria bassiana is a fungus which causes disease in insects. Currently it is used as an insecticide to control pest populations. Fungi are known to produce a vast array of secondary metabolites that are important for biotechnological applications. Furthemore, B. bassiana is an interesting source of biologically active molecules. There are alkaloids with the structure of 2-pyridone, dibenzoquinone pigments and different cyclodepsipeptides. Cyclodepsipeptides from B. bassiana are interesting for their neuroprotective properties. Interest of psychopharmacology is focused on the group of beauveriolides. Plant B. bassiana becomes a candidate for the prevention and treatment of neurodegenerative diseases.
Jaroslav Zdara, Petr Kral, Bozena Vasinova, Lada Kovarova
2016, 89 - 91
Military Training Area Libava was the venue for the 16th Military Medical Services Conference. This event has a long tradition and it became an integral part of military education actions. We can say, that the level of the conference is gradually improving year by year...
Vanda Bostikova, Kamil Kuca, Radek Sleha, Markéta Pasdiorova, Jan Marek, Hana Stritecka, Vladimir Hytych, Pavel Bostik
2016, 94 - 103
In February 2016, the World Health Organization (WHO) declared the current Zika outbreak a Public Health Emergency of international concern. The Zika virus disease is caused by a virus (family Flaviviridae) transmitted by Aedes spp. mosquitoes. People with the Zika virus disease usually have symptoms that can include a low-grade fever, maculopapular rash, conjunctivitis, arthralgia, malaise, headache and retro-ocular headaches. Neurological and autoimmune complications have been described during the outbreaks in Polynesia and recently in Brazil. There are no licensed medical countermeasures - vaccines, therapies or preventive drugs available for the Zika virus infection and disease.
EXPRESSION OF p21 AND EXPRESSION AND ACTIVATION OF MAPK REGULATED TRANSCRIPTION FACTORS IN PERIPHERAL BLOOD MONONUCLEAR CELLS IN RATS AFTER WHOLE-BODY γ-RADIATION AND ITS USE IN BIODOSIMETRY
Jaroslav Pejchal, Sarka Pruchova, Klara Kubelkova, Ales Tichy, Jirina Vavrova
2016, 104 - 110
The aim of our study was to examine the in vivo expression of p21 and expression and activation of ATF-2, c-Myc, and CREB in rat peripheral blood mononuclear cells (PBMC) after whole body γ-irradiation and to assess its contribution to biodosimetry. For Western blot experiments, male Wistar rats were whole-body irradiated by a single dose of 0, 0.5, 1, 3, and 5 Gy (60Co, 1 m, 0.7 Gy/min). As a positive control, leukaemic MOLT-4 cells were used. For ELISA experiments, male Wistar rats were whole-body irradiated by a single dose of 0, 1, 2, 3, 4, and 5 Gy (60Co, 1 m, 0.6 Gy/min). Blood samples were taken 4 h after the irradiation and PBMC were isolated using centrifugation on Histopaque-1077. Expressions of p21, ATF-2, phospho-ATF-2Thr69/71, c-Myc, phospho-c-MycThr-58/Ser62, CREB, and phospho-CREBSer133 were measured using Western blot method. Expression of p21 was also quantified using ELISA. We observed increase of p21 expression in rat PBMC 4 h after irradiation. According to ELISA, p21 levels increased 2.0-, 3.1-, 5.5-, 3.0-, and 3.1fold after irradiation by 1, 2, 3, 4, and 5 Gy, respectively. We did not detect any expression or activation of ATF-2, c-Myc and CREB. Protein p21 could be considered as a perspective biodosimetric marker of clinically significant irradiation (≥1 Gy) in vivo in unstimulated PBMC.
Haskova Katerina, Dyrhonova Marketa, Bostikova Vanda
2016, 111 - 120
Fecal microbiota therapy is going through its renaissance period. Even in ancient China, stool and its derivats were used for therapy of various diseases. Now thanks to new molecular methods a new knowledge about the intestinal microbiome and its interference with the human physiology, this method can be used for concrete therapy of disease.
Jaroslav Kacerovsky, Ladislav Jebavy, Jan M. Horacek
2016, 121 - 129
The purpose of this work is to give a brief overview on history and main directions of work at the Department of Military Internal Medicine (DMIM). Chiefs (commanders) of the department are presented in chronological order together with tasks they had to deal with during a given period of time. A close cooperation of the DMIM with civilian hospital departments is pointed out, mainly in the areas of therapeutic and preventive care together with education. A departmental contribution to the development of the Military Medical Service is specified in detail, particularly surgical and non-surgical casualties support.
2016, 130 - 131
Gulf War Syndrome (GWS), also known as Gulf War Illness (GWI), is a long-term multi-symptom disorder which was described in military veterans and civilian workers who were employed in the first Gulf War (1990–1991). Out of approximately 700 000 soldiers who served in Operation Desert Storm (ODS), one-fourth to one-third (25–32%) of them reported symptoms corresponding to GWS. Medically unexplained symptoms have been reported among both civilians and military personnel involved in the combat. A wide range of acute and chronic symptoms have been linked to it, including fatigue, muscle pain, cognitive problems, rashes and diarrhea. Twenty-five years after the end of the fighting, the mystery of GWS ...
2016, 133 - 136
2016, 138 - 138
Martin Jakl, Pavel Skorepa, Jan M. Horacek
2016, 139 - 146
Jiri Patocka, Kamil Kuca
2016, 147 - 163
Lead (Pb), which is widely used in industry, is a common element found in low concentrations in the Earth’s crust. Lead has been used since prehistoric times. As a highly toxic heavy metal, the pollution and exposure risks of lead are of widespread concern for human health. This environmental contaminant causing irreversible health effects mainly in children. Especially children under the age of six are most at risk for lead poisoning. Lead toxicity causes hematological, gastrointestinal, and neurological dysfunction. Symptoms are usually noted with blood lead greater than 2 μmol/litre. Severe or prolonged exposure may also cause chronic nephropathy, hypertension, and reproductive impairment. Lead inhibits some enzymes, alters cellular calcium metabolism, stimulates synthesis of binding proteins in kidney, brain, and bone, and slows down nerve conduction. The emission sources and pathological effects of lead are discussed.
Vanda Bostik, Radek Sleha, Miloslav Salavec, Sylva Janovska, Roman Chlibek, Pavel Blazek, Hana Stritecka, Vladislav Hytych, Kamil Kuca, Irena Hanovcova, Renata Sosovickova, Jan Smetana, Miroslav Splino, Jan Marek, Pavel Bostik
2016, 164 - 170
Jiri Patocka, Eugenie Nepovimova, Kamil Kuca
2016, 171 - 181
Short-chain unsaturated aliphatic alcohols exist as volatile liquids, some of them are used in both industrial and non-industrial environments, and some of them are natural products of different organisms. These alcohols very often work as semiochemicals, including acting as pheromones, attractants, repellents, etc.. Long-chain poly-unsaturated alcohols are often toxic principals of certain plants, thereby presenting a risk to humans.
2016, 182 - 185
THE 2ND INTERNATIONAL CONFERENCE CBRNE - RESEARCH & INNOVATION, LYON FRANCE, MAY 29TH TO JUNE 1ST 2017
2016, 186 - 187