In spite of the enormous quantity of information from genomic and proteomic analysis the data do not mirror precisely in vivo situation in respect to functional profile of cellular system studied. Generally, the function of molecular entities is strictly associated with their tertiary structure, subcellular localization inside individual cell compartments andbasically on the consensual sequences, which compose the functional domains of protein molecule. Moreover, the metabolic and signaling cellular cascades rely on the protein-protein interactions, which are alsoout of the scope of, so called “expression proteomics”. To overcome this gap, the construction of virtual proteome using the algorithms of bioinformatics and the systematic study of protein-protein interactions through the isolation of protein complexes called “cell-maps proteomics” precedes the creation of real bacterial proteome. Moreover, the proteomic study of isolated individual cellular compartments can bring more precise information of function-related protein expression.

Keywords: Francisella tularensis; Computer algorithms