VZL 2007, 76(2):56-59

Biosensors for Mycotoxins Assay

Miroslav Pohanka ORCID...1, Daniel Jun ORCID...2,3, Kamil Kuča ORCID...2,3
1 Ústřední vojenský zdravotní ústav, Centrum biologické ochrany, Těchonín
2 Univerzita obrany, Centrum pokročilých studií Fakulty vojenského zdravotnictví, Hradec Králové
3 Univerzita obrany, katedra toxikologie Fakulty vojenského zdravotnictví, Hradec Králové

Mykotoxiny jsou jedovaté sekundární metabolity produkované některými druhy hub. Mohou být přítomny v některých zaplísněných potravinách, není však možno vyloučit zneužití mykotoxinů v rámci teroristických aktivit. Včasná detekce a identifikace mykotoxinů hraje důležitou roli v ochraně před těmito látkami. Pro tyto účely bylo v minulosti vytvořeno několik komerčních aplikací metod, jako je HPLC nebo ELISA. Nicméně citlivá metoda využívající jednoduché přenosné zařízení s nízkými pořizovacími a provozními náklady stále není běžně dostupná. Biosenzory se zdají být vhodnou analytickou aplikací splňující vložená očekávání. V tomto souhrnném článku jsou popsány již existující biosenzory, jako jsou fluorimetrické array nebo SPR, spolu s těmi pro budoucnost perspektivními, jako jsou elektrochemické nebo piezoelektrické.

Keywords: Biosenzor; Imunosenzor; Mykotoxin

Mycotoxins are toxic fungal metabolites that can primary occur in contaminated food commodities. On the other side we can not exclude the risk of mycotoxins misuse for bioterrorist purposes. The ability of timely detection and identification of mycotoxins is one of the main tasks in protection activities. Several commercial methods for mycotoxins assays such as HPLC and/or ELISA were described. Nevertheless a sensitive method, using a simple, portable, and low cost device, has been still missing. Biosensors seem to be convenient methods for mycotoxin assay that fulfill noticed expectancies. In this article the existing biosensors such as fluorometric arrays or SPR biosensors likewise the promising ones such as electrochemical and piezoelectric based biosensors are described.

Keywords: Biosensor; Immunosensor; Mycotoxin

Received: January 22, 2007; Published: June 1, 2007  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Pohanka, M., Jun, D., & Kuča, K. (2007). Biosensors for Mycotoxins Assay. Vojenské Zdravotnické Listy, 76(2), 56-59
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. BRNDIAR, M. - KAČEROVSKÝ, J. - MALÍŘ, F., et al. Mykotoxiny jako bojové biologické látky? Voj. zdrav. Listy, 2003, roč. 72, č. 5, s. 203-207.
    2. CARLSON, MA. - BARGERON, CB. - BENSON, RC., et al. An automated, handheld biosensor for aflatoxin. Biosens. Bioelectron., 2000, vol. 14, no. 10, p. 841-848. Go to original source... Go to PubMed...
    3. DALY, SJ. - KEATING, GJ. - DILLON, PP., et al. Development of surface plasmon resonance-based immunoassay for aflatoxin B1. J. Agric. Food Chem., 2000, vol. 48, p. 5097-5104. Go to original source... Go to PubMed...
    4. GAAG, B. - SPATH, S. - DIETRICH, H., et al. Biosensors and multiple mycotoxin analysis. Food Cont., 2003, vol. 14, p. 251-254. Go to original source...
    5. LEE, WW. - THOMPSON, HG. - HALL, JG., et al. Rapid detection and identification of biological and chemical agents by immunoassays, gene probe assay and enzyme inhibition using a silicon-based biosensor. Biosens. Bioelectron., 2000, vol. 14, no. 10, p. 795-804. Go to original source... Go to PubMed...
    6. LIGLER, FS. - TAITT, CR. - SHRIVER-LAKE, LC., et al. Array biosensor for detection of toxins. Anal. Bioanal. Chem., 2003, vol. 377, no. 3, p. 469-477. Go to original source... Go to PubMed...
    7. LOGRIECO, A. - ARRIGAN, DW. - BRENGEL-PESCE, K., et al. DNA arrays, electronic noses and tongues, bio-sensors and receptors for rapid detection of toxigenic fungi and mycotoxins: a review. Food Addit. Contam., 2005, vol. 22, no. 4, p. 335-344. Go to original source... Go to PubMed...
    8. MAGAN, N. Mycotoxin contamination of food in Europe: early detection and prevention stragegies. Mycopathologia, 2006, vol. 162, no. 3, p. 245-253. Go to original source... Go to PubMed...
    9. MARAGOS, CM. Novel assays and sensor platforms for the detection of aflatoxins. Adv. Exp. Med. Biol., 2002, vol. 504, p. 85-93. Go to original source... Go to PubMed...
    10. NGUNDI, MM. - QADRI, SA. - WALLACE, EV., et al. Detection of deoxynivalenol in foods and indoor air using an array biosensor. Environ. Sci. Technol., 2006, vol. 40, p. 2352-2356. Go to original source... Go to PubMed...
    11. NGUNDI, MM. - SHRIVER-LAKE, LC. - MOORE, MH., et al. Array biosensor for detection of ochratoxin A in cereals and beverages. Anal. Chem., 2005, vol. 77, no. 1, p. 148-154. Go to original source... Go to PubMed...
    12. BERGER, U. - OEHME, M. - KUHN, F. Quantitative determination and structure elucidation of type A-and B-trichothecenes by HPLC/ion trap multiple mass spectrometry. J. Agric. Food Chem., 1999, vol. 47, no. 10, p. 4240-4245. Go to original source... Go to PubMed...
    13. RASOOLY, A. - HEROLD, KE. Biosensors for the analysis of food- and waterborne pathogens and their toxins. J. AOAC Int., 2006, vol. 89, no. 3, p. 873-883. Go to original source... Go to PubMed...
    14. SAPSFORD, KE. - TAITT, CR. - FERTIG, S., et al. Indirect competitive immunoassay for detection of aflatoxin B1 in corn and nut products using the array biosensor. Biosens. Bioelectron., 2006, vol. 21, no. 12, p. 2298-2305. Go to original source... Go to PubMed...
    15. SHONS, A. - DORMAN, F. - NAJARIAN, J. An immunospecific microbalance. J. Biomed. Mater. Res., 1972, vol. 6, p. 565-570. Go to original source... Go to PubMed...
    16. TURENER, NW. - PILETSKA, EV. - KARIM, K., et al. Effect of the solvent on recognition properties of moleculary imprinted polymer specific for ochratoxin A. Biosens. Bioelectron., 2004, vol. 20, no. 6, p. 1060-1067. Go to original source... Go to PubMed...
    17. WADKINS, RM. - GOLDEN, JP. - PRITSIOLAS, LM., et al. Detection of multiple toxic agents using a planar array immunosesor. Biosens. Bioelectron., 1998, vol. 13, no. 3, p. 407-415. Go to original source... Go to PubMed...
    18. ZHENG, MZ. - RICHARD, JL. - BINDER, J. A review of rapid methods for the analysis of mycotoxins. Mycopathologia, 2006, vol. 161, no. 5, p. 261-273. Go to original source... Go to PubMed...

    Return to the content