VZL 2008, 77(3):104-108

Proteome Analysis of Microorganism Borrelia garinii

Věra Neubauerová1, Aleš Macela ORCID...2, Lenka Hernychová2, Juraj Lenčo2, Martin Brychta2, Jana Havlasová2, Jan Kopecký3, Jitka Pechová3
1 Ústřední vojenský zdravotní ústav, Praha
2 Univerzita obrany, Ústav molekulární patologie Fakulty vojenského zdravotnictví, Hradec Králové
3 Parazitologický ústav Akademie věd ČR, České Budějovice

Lymeská borelióza je vážným onemocněním, jehož původcem je v Evropě nejčastěji Borrelia garinii. Pro pochopení patogeneze tohoto onemocnění je důležité získat informace o proteinovém složení původce a významným zdrojem těchto informací je proteomová analýza. Pro identifikaci pomocí dvojrozměrové elektroforézy a hmotnostní spektrometrie bylo vybráno 63 proteinových spot Borrelia garinii (kmen CB61). Z nich bylo úspěšně identifikováno 40, většina se však vyskytovala v různých izoformách. Identifikováno tak bylo celkem 16 různých proteinů. Převažují proteiny s enzymatickou funkcí, ale podařilo se také identifikovat některé proteiny zahrnuté v enzootickém cyklu. Jsou to zejména proteiny skupiny Osp, membránový protein p66 a flagelární protein.

Keywords: Borrelia garinii; Proteomová analýza; 2-DE; MALDI-TOF MS

Lyme disease is a serious illness caused in Europe most frequently by Borrelia garinii. It is important to find information about protein content for understanding pathogenesis of Lyme disease. Proteome analysis is an important source of this information. We used 2-dimensional electrophoresis and mass spectrometry for identification of 63 chosen Borrelia garinii (strain CB61) protein spots. Overall 40 protein spots were identified however most of them occurred in several isoforms. Totally 16 different proteins were identified. Among them we identified several enzymes. Proteins that participate in enzootic cycle of pathogen are more interesting. They are mainly proteins of Osp group, membrane protein p66 and flagellar protein.

Keywords: Borrelia garinii; Proteome analysis; 2-DE; MALDI-TOF MS

Received: July 11, 2008; Published: September 1, 2008  Show citation

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Neubauerová, V., Macela, A., Hernychová, L., Lenčo, J., Brychta, M., Havlasová, J., Kopecký, J., & Pechová, J. (2008). Proteome Analysis of Microorganism Borrelia garinii. Vojenské Zdravotnické Listy, 77(3), 104-108
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    References

    1. BARBOUR, AG. - TESSIER, SL. - HAYES, SF. Variation in a major surface protein on Lyme disease spirochetes. Infect. Immun., 1984, vol. 4, p. 94-100. Go to original source...
    2. BARBOUR, AG. Isolation and cultivation of Lyme disease spirochetes. Yale J. Biol. Med., 1984, vol. 57, p. 521-525. Go to PubMed...
    3. BARBOUR, AG. - HEILAND, RA. - HOWE, TR. Heterogenity of Major Proteins in Lyme Disease Borreliae: Molecular Analysis of North American and European Isolates. J. Infect. Dis., 1985, vol. 152, p. 478-484. Go to original source... Go to PubMed...
    4. BERGSTRÖM, S., et al. Molecular and Cellular Biology of Borrelia burgdorferi sensu lato. In Lyme Borreliosis: Biology, Epidemiology and Control. UK, CAB International, 2002, p. 47-90. Go to original source...
    5. BOUCHAL, P. - KUČERA, I. Dvourozměrná elektroforéza v proteomice: principy a aplikace. Chem. Listy, 2003, roč. 97, s. 29-36.
    6. BRUSCA, JS., et al. Localization of outer surface proteins A and B in both the outer membrane and intracellular compartments of Borrelia burgdorferi. J. Bacteriol., 1991, vol. 173, p. 8004-8008. Go to original source... Go to PubMed...
    7. BUNIKIS, J. - NOPPA, L. - BERGSTRÖM, S. Molecular analysis of a 66-kDa protein associated with the outer membrane of Lyme disease Borrelia. FEMS Microbiol. Let., 1995, vol. 131, p. 139-145. Go to original source... Go to PubMed...
    8. GOLDSTEIN, SF. - BUTTLE, KF. - CHARON, NW. Structural Analysis of the Leptospiraceae and Borrelia burgdorferi by High- Voltage Electron Microscopy. J. Bacteriol., 1996, vol. 178, p. 6539-6545. Go to original source... Go to PubMed...
    9. GÖRG, A., et al. Very alkaline immobilized pH gradients for two-dimensional electrophoresis of ribosomal and nuclear proteins. Electrophoresis, 1997, vol. 18, p. 328-337. Go to original source... Go to PubMed...
    10. HAVLASOVÁ, J., et al. Proteomic analysis of anti-Francisella tularensis LVS antibody response in murine model of tularemia. Proteomics, 2005, vol. 5, p. 2090-2103. Go to original source... Go to PubMed...
    11. HOCHSTRASSER, DF. - MERRIL, CR. Catalysis for polyacrylamide gel polymerization and detection of proteins by silver staining. Appl. Theor. Electrophor., 1988, vol. 1, p. 35-40. Go to PubMed...
    12. HUBÁLEK, Z. - HALOUZKA, J. Distribution of Borrelia burgdorferi sensu lato genomic groups in Europe (Review). Eur. J. Epidemiol., 1997, vol. 13, p. 951-957. Go to original source... Go to PubMed...
    13. HUBALEK, M., et al. Comparative proteome analysis of the cellular proteins extracted from highly virulent Francisella tularensis ssp. tularensis and less virulent F. tularensis ssp. holarctica and F. tularensis ssp. mediaasiatica. Proteomics, 2004, vol. 4, p. 3048-3060. Go to original source... Go to PubMed...
    14. CHARON, NW., et al. Spirochete chemotaxis, motility, and the structure of the spirochetal periplasmic flagella. Res. Microbiol., 1992, vol. 143, p. 597-603. Go to original source... Go to PubMed...
    15. JUNGBLUT, PR., et al. Proteomics in human disease: Cancer, heart and infectious diseases. Electrophoresis, 1999, vol. 20, p. 2100-2110. Go to original source...
    16. JUNGBLUT, P. - GRABHER, G. - STÖFFLER, G. Comprehensive detection of Immunorelevant Borrelia garnii antigens by two-dimensional electrophoresis. Electrophoresis, 1999, vol. 20, p. 3611-3622. Go to original source... Go to PubMed...
    17. KIMSEY, RB. - SPIELMAN, A. Motility of Lyme disease spirochetes in fluids as viscous as the extracellular matrix. J. Infec. Dis., 1990, vol. 162, p. 1205-1208. Go to original source... Go to PubMed...
    18. LENCO, J., et al. Insights into the oxidative stress response in Francisella tularensis LVS and its mutant ∆iglC1 + 2 by proteomics analysis. FEMS Microbiol. Lett., 2005, vol. 246, p. 47-54. Go to original source... Go to PubMed...
    19. MAGNUSSON, HJ. - HILL, C. - FLEISCHMAN, R. The minimal infectious inoculum of Spirochaeta pallida (Nichols strain) and a consideration of its rate of multiplication in vivo. Am. J. Syphilis, Gonorrhea Venerol. Dis., 1948, vol. 32, p. 1-18.
    20. NOWALK, AJ., et al. Comparative proteome analysis of subcellular fractions from Borrelia burgdorferi by NEPHGE and IPG. Proteomics, 2006, vol. 6, p. 2121-2134. Go to original source... Go to PubMed...
    21. O'CONNELL, S., et al. Epidemiology of European Lyme borreliosis. Zentralbl. Bakteriol., 1998, vol. 287, p. 229-240. Go to original source... Go to PubMed...
    22. PAL, U., et al. Attachment of Borrelia burgdorferi within Ixodes scapularis mediated by outer surface protein A. J. Clin. Investig., 2000, vol. 106, p. 561-569. Go to original source... Go to PubMed...
    23. RADOLF, JD., et al. Characterization of Outer Membranes Isolated from Borrelia Burgdorferi, the Lyme Disease Spirochete. Infect. Immun., 1995, vol. 63, p. 2154-2163. Go to original source... Go to PubMed...
    24. SADZIENE, A., et al. A flagella-less mutant of Borrelia burgdorferi. Structural, molecular, and in vitro functional characterization. J. Clin. Investig., 1991, vol. 88, p. 82-92. Go to original source... Go to PubMed...
    25. SCHWAN, TG. - PIESMAN, J. Temporal Changes in Outer Surface Proteins A and C of the Lyme Disease-Associated Spirochete, Borrelia burgdorferi, during the Chain of Infection in Ticks and Mice. J. Clin. Microbiol., 2000, vol. 38, p. 382-388. Go to PubMed...
    26. SKARE, JT., et al. The Oms66 (p66) Protein Is a Borrelia burgdorferi Porin. Infect. Immun., 1997, vol. 65, p. 3654-3661. Go to original source... Go to PubMed...
    27. STEERE, AC. Lyme disease: A growing threat to urban populations. Proceedings of the National Academy of Science, USA, 1994, vol. 91, p. 2378-2383. Go to original source... Go to PubMed...
    28. Swiss-Prot Protein Knowledgebase (ExPASy) [databáze online]. Swiss Institute of Bioinformatics [citováno 2006-03-17]. Dostupné z: http://br.expasy.org/uniprot/
    29. TIGR-CMR [databáze online]. The Comprehensive Microbial Resource Database [citováno 2006-08-07]. Dostupné z: http://cmr.tigr.org/tigrscripts/CMR/CmrHomePage.cgi
    30. Integrated microbial genomes [databáze online]. Doe Joint Genome Institute [citováno 2006-08-07]. Dostupné z: http://img.jgi.doe.gov/cgi-bin/pub/main.cgi
    31. EMBL-EBI Database [databáze online]. The European Molecular Biology Laboratory - The European Bioinformatics Institute [citováno 2006-03-17]. Dostupné z: http://www.ebi.ac.uk/interpro

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