MMSL 2017, 86(3):96-103 | DOI: 10.31482/mmsl.2017.017

ANALYSIS OF NERVE AGENTS THROUGH A MODIFIED METHOD OF CHOLINESTERASE ASSESSMENT USING ARTIFICIAL NEURONAL NETWORKSOriginal article

Monika Hoskovcová1*, Pavel Dubina2, Emil Halámek1, Zbyněk Kobliha1
1 NBC Defence Institute, University of Defence Brno, Vita Nejedleho, Vyskov, 682 01, Czech Republic
2 Military Research Institute, Veslarska 230, Brno 637 00, Czech Republic

A simple colorimetric biosensor, which uses the modified Ellman's reaction, enables selective analysis of nerve agents based on a different ability of bispyridinium oximes to reactivate enzyme-inhibitor complexes in the phase before dealkylation. The analysis was made based on spectral data of reflectance of the surface of a cotton cloth reaction zone of biosensor with immobilized and stabilized enzyme after inhibition and subsequent reactivation. The evaluation of measured data was made based on a method of artificial neuronal networks. The individual inhibitors from the groups of three nerve agents were identified: sarin, soman, tabun, cyclosarin, agent VX and its Russian analogue, R-33.

Keywords: Ellman's reagent; chemical warfare agent; colorimetric biosensor; acetylcholinesterase reactivator; neural analysis

Received: May 15, 2017; Revised: June 9, 2017; Published: September 8, 2017  Show citation

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Hoskovcová, M., Dubina, P., Halámek, E., & Kobliha, Z. (2017). ANALYSIS OF NERVE AGENTS THROUGH A MODIFIED METHOD OF CHOLINESTERASE ASSESSMENT USING ARTIFICIAL NEURONAL NETWORKS. MMSL, 86(3), 96-103. doi: 10.31482/mmsl.2017.017
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