MMSL 2020, 89(3):126-141 | DOI: 10.31482/mmsl.2020.011

OPCW BIOMEDICAL PROFICIENCY TEST IN THE LABORATORY OF ANALYTICAL CHEMISTRY AT THE DEPARTMENT OF TOXICOLOGY AND MILITARY PHARMACYOriginal article

David Herman ORCID...1*, Alžběta Dlabková ORCID...1, Nela Váňová ORCID...1,2, Lukáš Prchal ORCID...1,3, Marie Vajrychová ORCID...1,3, Rafael Doležal ORCID...1,3, Petr Bzonek ORCID...1, Daniel Jun ORCID...1
1 Katedra toxikologie a vojenské farmacie, Fakulta vojenského zdravotnictví, Univerzita obrany, Třebešská 1575, 500 02, Hradec Králové, Česká republika
2 Katedra farmaceutické chemie a farmaceutické analýzy, Farmaceutická fakulta, Univerzita Karlova, Akademika Heyrovského 1203, 500 05 Hradec Králové, Česká republika
3 Centrum biomedicínského výzkumu, Fakultní nemocnice Hradec Králové, Sokolská 581, 500 05 Hradec Králové, Česká republika

S ohledem na události posledních let není riziko vojenského či teroristického zneužití vysoce toxických látek včetně zakázaných bojových otravných látek zanedbatelné. Katedra toxikologie a vojenské farmacie Fakulty vojenského zdravotnictví Univerzity obrany tudíž zavedla analytické metody a postupy úprav biologických vzorků (plasma, moč) pro stanovení identifikačních znaků (biomarkerů) vystavení nervově paralytickým látkám (sarinu, cyklosarinu, somanu, tabunu a V-látkám) a yperitu, čímž přináší do Armády České republiky schopnost potvrdit jejich zneužití vůči živé síle. Metody popsané v článku jsou založeny na průkazu metabolitů (O-alkyl methylfosfonových kyselin pro nervově paralytické látky a thiodiglykolu pro yperit) a produktů jejich reakcí s přítomnými biomolekulami (tyrosinových aduktů nervově paralytických látek a peptidových aduktů sirného yperitu) pomocí kapalinové a plynové chromatografie spojené s hmotnostní spektrometrií. Funkčnost těchto metod je ověřována a diskutována v rámci testu odborné způsobilosti pro biomedicínskou analýzu s mezinárodní účastí, který každoročně pořádá Organizace pro zákaz chemických zbraní. Katedra se těchto testů účastní od roku 2016, kdy se konal první ročník.

Keywords: chemické bojové látky; yperit; OPCW; BioPT; biomarkery

Taking into account the events of recent years, a risk of exposure to high toxic substances including chemical weapons is not negligible. Therefore the Department of Toxicology and Military Pharmacy, Faculty of Military Health Sciences, University of Defence introduced analytical and biological samples (plasma, urine) preparation methods for determination of biomarkers of exposure to nerve agent (sarin, cyclosarin, soman, tabun, VX and VR agent) and sulphur mustard. Evidence of chemical weapon use is based on detection of their metabolites (Alkyl methylphosphonic acids as metabolites of the organophosphorus nerve agents, thiodiglycol as a metabolite of sulphur mustard) and products of their reactions with biomolecules (tyrosine adducts of organophosphorus nerve agents, sulphur mustard-albumin adducts). Gas chromatography tandem mass spectrometry and liquid chromatography tandem mass spectrometry are used for determination of biomarkers. Functionality of described methods is validated and discussed participating in Biomedical proficiency test, which is organized by The Organisation for the Prohibition of Chemical Weapons every year.

Keywords: chemical warfare agent; sulphur mustard; OPCW; BioPT; biomarkers

Received: April 24, 2020; Revised: May 7, 2020; Accepted: May 7, 2020; Prepublished online: May 22, 2020; Published: September 4, 2020  Show citation

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Herman, D., Dlabková, A., Váňová, N., Prchal, L., Vajrychová, M., Doležal, R., Bzonek, P., & Jun, D. (2020). OPCW BIOMEDICAL PROFICIENCY TEST IN THE LABORATORY OF ANALYTICAL CHEMISTRY AT THE DEPARTMENT OF TOXICOLOGY AND MILITARY PHARMACY. MMSL, 89(3), 126-141. doi: 10.31482/mmsl.2020.011
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