MMSL 2022, 91(4):335-342 | DOI: 10.31482/mmsl.2022.011

VARIATION OF CALCIUM DISTRIBUTION IN DIFFERENT ORGANS OF LIVER FLUKE FASCIOLA HEPATICAOriginal article

H.S. Sadoon ORCID...1*, Buthaina H. Al-Sabawi ORCID...2, Sundus N. Al-Kallak ORCID...3
1 College of Education for Pure Science, University of Mosul, Mosul, Iraq
2 College of Medicine, University of Mosul, Mosul, Iraq
3 College of Science, University of Mosul, Mosul, Iraq

Introduction: Livestock, including sheep, are infected with many types of parasitic diseases, and among these diseases is fascioliasis, or the so-called liver rot, which is caused by the Fasciola hepatica worm. This disease is widespread in many regions of the world, especially those with moderate climatic conditions. This disease harms the state’s economy and livestock breeders due to the high rates of infection, which greatly affects animal production.

Materials: Wax sections were made from F. hepatica, cut with a thickness of 7 microns, and stained with Hematoxylin and Eosin stain and Von Kossa technique to investigate the calcium sites in different organs of the worm.

Results: There was a clear discrepancy in the density of calcium present in several internal organs of F. hepatica.

Conclusion: We infer from this research that there is a clear variation in the density of calcium as well as the size and shape of the calcareous corpuscles in their different locations in the worm’s organs.

Keywords: parasites; Fasciola hepatica; calcium; minerals; Calcareous corpuscles

Received: January 8, 2022; Revised: February 1, 2022; Accepted: February 1, 2022; Prepublished online: March 17, 2022; Published: December 2, 2022  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Sadoon, H.S., Al-Sabawi, B.H., & Al-Kallak, S.N. (2022). VARIATION OF CALCIUM DISTRIBUTION IN DIFFERENT ORGANS OF LIVER FLUKE FASCIOLA HEPATICA. MMSL91(4), 335-342. doi: 10.31482/mmsl.2022.011
Download citation

References

  1. Mas-Coma S, Funatsu IR, Bargues MD. Fasciola hepatica and lymnaeid snails occur at very high altitude in South America. Parasitology. 2001 Nov;123(7):115-27. https://doi.org/10.1017/S0031182001008034 Go to original source... Go to PubMed...
  2. Mas-Coma S, Bargues MD, Valero MA. Fascioliasis and other plant-borne trematode zoonoses. International journal for parasitology. 2005 Oct 1;35(11-12):1255-78. https://doi.org/10.1016/j.ijpara.2005.07.010 Go to original source... Go to PubMed...
  3. Mas-Coma S. Epidemiology of fascioliasis in human endemic areas. Journal of helminthology. 2005 Sep;79(3):207-16. https://doi.org/10.1079/JOH2005296 Go to original source... Go to PubMed...
  4. Erasmus DA, Popiel I, Shaw JR. A comparative study of the vitelline cell in Schistosoma mansoni, S. haematobium, S. japonicum and S. mattheei. Parasitology. 1982 Apr;84(2):283-7. https://doi.org/10.1017/S0031182000044838 Go to original source... Go to PubMed...
  5. Smyth JD, McManus DP. The physiology and biochemistry of cestodes. Cambridge university press; 1989. Go to original source...
  6. Lumsden RD. The fine structure of adult tapeworms. Biology of the Eucestoda. 1983;1:208-18.
  7. McCullough JS, Fairweather I. The structure, composition, formation and possible functions of calcareous corpuscles in Trilocularia acanthiaevulgaris Olsson 1867 (Cestoda, Tetraphyllidea). Parasitology research. 1987 Mar;74(2):175-82. https://doi.org/10.1007/BF00536030 Go to original source... Go to PubMed...
  8. Etges FJ, Marinakis V. Formation and excretion of calcareous bodies by the metacestode (tetrathyridium) of Mesocestoides vogae. The Journal of parasitology. 1991 Aug 1:595-602. https://doi.org/10.2307/3283166 Go to original source...
  9. von Brand T, Weinbach EC. Incorporation of calcium into the soft tissues and calcareous corpuscles of larval Taenia taeniaeformis. Zeitschrift für Parasitenkunde. 1975 Mar;48(1):53-63. https://doi.org/10.1007/BF00389829 Go to original source... Go to PubMed...
  10. Desser SS. Calcium accumulation in larval echinococcus multilocularis with some observations on the leucocyte counts in infected rodents.
  11. Vargas-Parada LA, Laclette JP. Role of calcareous corpuscles in cestode physiology. Revista latinoamericana de microbiologia. 1999;41:303-7. Go to PubMed...
  12. Iamshanova O, Fiorio Pla A, Prevarskaya N. Molecular mechanisms of tumour invasion: Regulation by calcium signals. The Journal of physiology. 2017 May 15;595(10):3063-75. https://doi.org/10.1113/JP272844 Go to original source... Go to PubMed...
  13. Rondelaud D, Hourdin P, Vignoles P, et al. The contamination of wild watercress with Fasciola hepatica in central France depends on the ability of several lymnaeid snails to migrate upstream towards the beds. Parasitology research. 2005 Mar;95(5):305-9. https://doi.org/10.1007/s00436-004-1283-5 Go to original source... Go to PubMed...
  14. Chowdhury N, De Rycke PH. A new approach for studies on calcareous corpuscles in Hymenolepis microstoma. Zeitschrift für Parasitenkunde. 1974 Jun;43(2):99-103. https://doi.org/10.1007/BF00329167 Go to original source... Go to PubMed...
  15. Al-Kallak SN. Morphological, Histological and Chemical Studies on Two Cestode Model asFishParasites.2001; PhD thesis, College of Science, University of Mosul.(in Arabic).
  16. Al-Hayali FK. Histological and histochemical study of the walls of the hydatid cyst of Echinococcus granulosus of humans and some other intermediate hosts.1999;Master's Thesis, College f Science, University of Mosul. (inArabic)
  17. Al-Hansaniani JMA. Dignostic and pathological study of Fasciola hepatica infection in goats in Nineveh Governorate .2002; Master's Thesis, College of Veterinary Medicine, University of Mosul.(in Arabic)
  18. Al-Kallak SN, Al-Sabawi BH, Hameed SI. Study of calcareous corpuscles in liver worm Fasciola hepatica (Platyhelminthes:Trematoda) in Nineveh Governorate. Journal of Education and science. 2006;18(2):52-63. (In Arabic). Go to original source...
  19. Al-Kallak SN. Determination of polymorphism location of the calcareous corpuscles in the tapeworm Bothriocephalus acheillognathi. IBN AL-HAITHAM j. FOR PURE &APPL. SCI.2010; 23(3).
  20. Al-Kallak SN, Al-Niaeemi BH, Al-Sabawi BH. Histological study of the distribution of calcareous corpuscles and their role in tapeworm Amphicotyle heteropleura (Desind, 1850). Basra Research journal.2012;B-2(38):24-33. (in Arabic).
  21. Z Arafa S, S Abd El-Atti M, M Elraey S. Fine tegumental structures of the bothriocephalidean cestode, Oncodiscus sauridae, an intestinal parasite of the lizardfish Saurida undosquamis in Suez Gulf, Egypt. Egyptian Journal of Aquatic Biology and Fisheries. 2020 Mar 1;24(2):379-93. DOI: 10.21608/ejabf.2020.82261 Go to original source...
  22. Chowdhury N, De Rycke PH. Structure, formation, and functions of calcareous corpuscles in Hymenolepis microstoma. Zeitschrift für Parasitenkunde. 1977 Jan;53(2):159-69. https://doi.org/10.1007/BF00380461 Go to original source... Go to PubMed...
  23. Smith SA, Richards KS. Ultrastructure and microanalyses of the calcareous corpuscles of the protoscoleces of Echinococcus granulosus. Parasitology research. 1993 Mar;79(3):245-50. https://doi.org/10.1007/BF00931900 Go to original source... Go to PubMed...
  24. Tian X, Zhang B. Ultrastructural observations on the formation and metabolism of calcareous corpuscles in Cysticercus cellulosae. Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi= Chinese journal of parasitology & parasitic diseases. 1998 Jan 1;16(5):364-7.
  25. Varga-Parada L, Merchant MT, Willms K, et al. Formation of calcareous corpuscles in the lumen of excretory canals of Taenia solium cysticerci. Parasitology research. 1999 Feb;85(2):88-92. https://doi.org/10.1007/s004360050514 Go to original source... Go to PubMed...
  26. Vargas-Parada LA, Laclette JP. Role of calcareous corpuscles in cestode physiology. Revista latinoamericana de microbiologia. 1999;41:303-7. Go to PubMed...
  27. Ahn CS, Kim JG, Bae YA, et al. Fasciclin-calcareous corpuscle binary complex mediated protein-protein interactions in Taenia solium metacestode. Parasites & vectors. 2017 Dec;10(1):1-3. https://doi.org/10.1186/s13071-017-2359-2 Go to original source... Go to PubMed...
  28. Mehlhorn H. Encyclopedia of Parasitology. Springer-Verlag Berlin Heidelberg. New York (eds). 2008. https: 2008; doi.org/10.1007/978-3-540-48996-2_487.
  29. Graham MK, McGeown JG, Fairweather I. Ionic mechanisms underlying spontaneous muscle contractions in the liver fluke, Fasciola hepatica. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. 1999 Aug 1;277(2):R374-83. https://doi.org/10.1152/ajpregu.1999.277.2.R374 Go to original source... Go to PubMed...
  30. Mackiewicz JS, Ehrenpris MB. Calcareous corpuscles distribution in caryophyllid cestodes: possible evidence of cryptic segmentation. Proceedings of the Helminthological Society of Washington. 1980 Jan 1;47(1):1-9.
  31. Kennedy CR. Ecological aspects of parasitology. North-Holland Publishers; 1976.
  32. Cuesta-Herranz J, de las Heras M, Fernández M, et al. Allergic reaction caused by local anesthetic agents belonging to the amide group. Journal of allergy and clinical immunology. 1997 Mar 1;99(3):427-8. https://doi.org/10.1016/S0091-6749(97)70064-2 Go to original source... Go to PubMed...
  33. Subpipattana P, Grams R, Vichasri-Grams S. Analysis of a calcium-binding EF-hand protein family in Fasciola gigantica. Experimental parasitology. 2012 Apr 1;130(4):364-73. https://doi.org/10.1016/j.exppara.2012.02.005 Go to original source... Go to PubMed...
  34. Fitzsimmons CM, Jones FM, Stearn A, et al. The Schistosoma mansoni tegumental-allergen-like (TAL) protein family: influence of developmental expression on human IgE responses. PLoS neglected tropical diseases. 2012 Apr 3;6(4):e1593. https://doi.org/10.1371/journal.pntd.0001593 Go to original source...
  35. Kim YJ, Yoo WG, Lee MR, et al. Identification and characterization of a novel 21.6-kDa tegumental protein from Clonorchis sinensis. Parasitology research. 2012 May;110(5):2061-6. https://doi.org/10.1007/s00436-011-2681-0 Go to original source... Go to PubMed...
  36. Senawong G, Laha T, Loukas A, et al. Cloning, expression, and characterization of a novel Opisthorchis viverrini calcium-binding EF-hand protein. Parasitology international. 2012 Mar 1;61(1):94-100. https://doi.org/10.1016/j.parint.2011.07.012 Go to original source... Go to PubMed...
  37. Zheng Y, Guo X, Su M, et al. Identification of emu-TegP11, an EF-hand domain-containing tegumental protein of Echinococcus multilocularis. Veterinary parasitology. 2018 May 15;255:107-13. https://doi.org/10.1016/j.vetpar.2018.04.006 Go to original source... Go to PubMed...
  38. Jones MK, Gobert GN, Zhang L, et al. The cytoskeleton and motor proteins of human schistosomes and their roles in surface maintenance and host-parasite interactions. Bioessays. 2004 Jul;26(7):752-65. https://doi.org/10.1002/bies.20058 Go to original source... Go to PubMed...
  39. Huang SY, Yue DM, Hou JL, et al. Proteomic analysis of Fasciola gigantica excretory and secretory products (FgESPs) interacting with buffalo serum of different infection periods by shotgun LC-MS/MS. Parasitology research. 2019 Feb;118(2):453-60. https://doi.org/10.1007/s00436-018-6169-z Go to original source... Go to PubMed...
  40. Russell SL, Timson DJ. Calcium binding proteins in the liver fluke, Fasciola hepatica. InNew Developments in Calcium Signaling Research 2014 Jan (pp. 89-104). Nova Science Publishers, Inc.
  41. Flores-Bautista J, Navarrete-Perea J, Fragoso G, et al. Fate of uptaken host proteins in Taenia solium and Taenia crassiceps cysticerci. Bioscience reports. 2018 Jul 6;38(4):BSR20180636. https://doi.org/10.1042/BSR20180636 Go to original source... Go to PubMed...
  42. Fairweather I, Threadgold LT, Hanna RE. Development of Fasciola hepatica in the mammalian host. InFasciolosis 1999 (pp. 47-111). CAB International.
  43. Haçariz O, Sayers G, Mulcahy G. A preliminary study to understand the effect of Fasciola hepatica tegument on naïve macrophages and humoral responses in an ovine model. Veterinary immunology and immunopathology. 2011 Feb 15;139(2-4):245-9. https://doi.org/10.1016/j.vetimm.2010.08.007 Go to original source... Go to PubMed...
  44. Ravidà A, Cwiklinski K, Aldridge AM, et al. Fasciola hepatica surface tegument: glycoproteins at the interface of parasite and host. Molecular & Cellular Proteomics. 2016 Oct 1;15(10):3139-53. https://doi.org/10.1074/mcp.M116.059774 Go to original source... Go to PubMed...
  45. Wilson RA, Wright JM, de Castro-Borges W, et al. Exploring the Fasciola hepatica tegument proteome. International journal for parasitology. 2011 Nov 1;41(13-14):1347-59. https://doi.org/10.1016/j.ijpara.2011.08.003 Go to original source... Go to PubMed...
  46. Threadgold LT. Fasciola hepatica: ultrastructure and histochemistry of the glycocalyx of the tegument. Experimental parasitology. 1976 Feb 1;39(1):119-34. https://doi.org/10.1016/0014-4894(76)90019-9 Go to original source... Go to PubMed...
  47. Prasanphanich NS, Mickum ML, Heimburg-Molinaro J, et al. Glycoconjugates in host-helminth interactions. Frontiers in immunology. 2013 Aug 28;4:240. https://doi.org/10.3389/fimmu.2013.00240 Go to original source... Go to PubMed...
  48. Meevissen MH, Yazdanbakhsh M, Hokke CH. Schistosoma mansoni egg glycoproteins and C-type lectins of host immune cells: molecular partners that shape immune responses. Experimental parasitology. 2012 Sep 1;132(1):14-21. https://doi.org/10.1016/j.exppara.2011.05.005 Go to original source... Go to PubMed...
  49. Señorale-Pose M, Chalar C, Dauphin Y, et al. Monohydrocalcite in calcareous corpuscles of Mesocestoides corti. Experimental parasitology. 2008 Jan 1;118(1):54-8. Go to original source... Go to PubMed...