MMSL 2023, 92(1):87-94 | DOI: 10.31482/mmsl.2022.034

PEROXIREDOXIN 3 AND OXIDATIVE STRESS IN RECURRENT ABORTION PATIENTSOriginal article

Israa H. AL-Hamdani ORCID...1*, Luay A. Al-Helaly ORCID...2
1 Department of Basic Science, College of Dentistry, University of Mosul, Mosul, Iraq
2 Department of Chemistry, College of Science, University of Mosul, Mosul, Iraq

The pаthоphysiolоgy of recurrent spontaneous abortion (RSA) has been linked to oxidative stress (OS), which is defined as an imbalance between the formation of oxidants and the antioxidant defense system. The goal of this study was to assess the state of OS in recurrent spontaneous abortion by assessing some of its indicators in order to identify women who are at risk of abortion and enhance their reproductive health. Peroxiredoxin 3 (Prx3), progesterone (P4), glutathione (GSH), uric acid (UA), pеrоxynitritе (ONOO-), and mаlоndialdеhyde (MDA) were measured in 40 healthy non-pregnant (HNP) women, 40 healthy pregnant (HP) women without abortion history, and 21 women with recurrent spontaneous abortion (Have at least ≥ 3 consecutive abortion). All subjects are of reproductive age, with the mother gestational age in the HP and RSA groups being ≤ 20 weeks. According to maternal gestational age, RSA and HP women were separated into two categories (1st & 2nd trimester). According to the findings of this study, Prx3 and GSH levels declined considerably in RSA patients compared to HP and HNP patients, but ONOO- and MDA levels increased statistically significantly in RSA patients compared to HP and HNP groups. However, P4 of RSA was found to be significantly lower in the HP group and much higher in the HNP group. The difference in uric acid levels between the RSA and HP groups was statistically significant, but the difference between the RSA and HNP groups was not.Within the first and second trimesters of pregnancy, the difference between the RSA and HP groups showed statistically significant changes in oxidative stress-related biomarkers, with the exception of uric acid, which showed a non-significant difference between the two groups within the second trimester.Finally, the effect of gestational age within RSA was revealed, with serum Prx3 and P4 showing a significant difference between the first and second stages of pregnancy, whereas other oxidative stress indicators were unaffected by pregnancy length within the RSA group.

Keywords: Recurrent abortion; Oxidative stress; Antioxidants; Oxidants; Pеroxirеdоxin 3; Progesterone; Glutathione; Uric acid; Pеrоxynitritе; Malоndiаldеhydе

Received: May 30, 2022; Revised: July 25, 2022; Accepted: July 25, 2022; Prepublished online: January 5, 2023; Published: March 3, 2023  Show citation

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AL-Hamdani, I.H., & Al-Helaly, L.A. (2023). PEROXIREDOXIN 3 AND OXIDATIVE STRESS IN RECURRENT ABORTION PATIENTS. MMSL92(1), 87-94. doi: 10.31482/mmsl.2022.034
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References

  1. Quenby S, Gallos ID, Dhillon-Smith RK, et al. Miscarriage matters: the epidemiological, physical, psychological, and economic costs of early pregnancy loss. Lancet. 2021 May 1;397(10285):1658-1667. doi: 10.1016/S0140-6736(21)00682-6 Go to original source... Go to PubMed...
  2. Alves C, Rapp A. StatPearls [Internet]. StatPearls Publishing; Treasure Island (FL): Jul 20, 2021. Spontaneous Abortion. [PubMed]
  3. Dimitriadis E, Menkhorst E, Saito S, et al. Recurrent pregnancy loss. Nature reviews disease primers. 2020 Dec 10;6(1):1-9. Go to original source... Go to PubMed...
  4. Lathi RB, Gray Hazard FK, Heerema-McKenney A, et al. First trimester miscarriage evaluation. Semin Reprod Med. 2011 Nov;29(6):463-469. doi: 10.1055/s-0031-1293200 Go to original source... Go to PubMed...
  5. Kumar S. Occupational, environmental and lifestyle factors associated with spontaneous abortion. Reprod Sci. 2011 Oct;18(10):915-30. doi: 10.1177/1933719111413298 Go to original source... Go to PubMed...
  6. Torkzahrani S, Ataei PJ, Hedayati M, et al. Oxidative stress markers in early pregnancy loss: a case-control study. International Journal of Women's Health and Reproduction Sciences. 2019 Jan 1;7:61-66. Go to original source...
  7. Bazmi S, Behnoush B, Kiani M, et al. Comparative Study of Therapeutic Abortion Permissions in Central Clinical Department of Tehran Legal Medicine Organization before and after Approval of Law on Abortion in Iran. Iran J Pediatr. 2008;18(4):315-322.
  8. Zejnullahu VA, Zejnullahu VA, Kosumi E. The role of oxidative stress in patients with recurrent pregnancy loss: a review. Reprod Health. 2021 Oct 16;18(1):207. doi: 10.1186/s12978-021-01257-x Go to original source... Go to PubMed...
  9. Azizi R, Soltani-Zangbar MS, Sheikhansari G, et al. Metabolic syndrome mediates inflammatory and oxidative stress responses in patients with recurrent pregnancy loss. J Reprod Immunol. 2019 Jun;133:18-26. doi: 10.1016/j.jri.2019.05.001 Go to original source... Go to PubMed...
  10. ESHRE Guideline Group on RPL, Bender Atik R, Christiansen OB, et al. ESHRE guideline: recurrent pregnancy loss. Human Reproduction Open. 2018;2018(2):hoy004. Go to original source... Go to PubMed...
  11. Pandey MK, Rani R, Agrawal S. An update in recurrent spontaneous abortion. Arch Gynecol Obstet. 2005 Jul;272(2):95-108. doi: 10.1007/s00404-004-0706-y Go to original source... Go to PubMed...
  12. Dendrinos S, Makrakis E, Botsis D, et al. A study of pregnancy loss in 352 women with recurrent miscarriages. Arch Gynecol Obstet. 2005 Mar;271(3):235-239. doi: 10.1007/s00404-004-0607-0 Go to original source... Go to PubMed...
  13. Gupta S, Agarwal A, Banerjee J, et al. The role of oxidative stress in spontaneous abortion and recurrent pregnancy loss: a systematic review. Obstet Gynecol Surv. 2007 May;62(5):335-347. doi: 10.1097/01.ogx.0000261644.89300.df Go to original source... Go to PubMed...
  14. Hussain T, Murtaza G, Metwally E, et al. The Role of Oxidative Stress and Antioxidant Balance in Pregnancy. Mediators Inflamm. 2021 Sep 27; 2021: 9962860. doi: 10.1155/2021/9962860 Go to original source... Go to PubMed...
  15. Bilici M. The Importance of Oxidative Stress in Early Week Pregnancy Losses. Crescent J Med Biol Sci. 2014;1(4):151-153.
  16. Chae HZ, Chung SJ, Rhee SG. Thioredoxin-dependent peroxide reductase from yeast. J Biol Chem. 1994 Nov 4;269(44):27670-27678. Go to original source...
  17. Nelson KJ, Knutson ST, Soito L, et al. Analysis of the peroxiredoxin family: using active-site structure and sequence information for global classification and residue analysis. Proteins. 2011 Mar;79(3):947-64. doi: 10.1002/prot.22936 Go to original source... Go to PubMed...
  18. Cox AG, Winterbourn CC, Hampton MB. Mitochondrial peroxiredoxin involvement in antioxidant defence and redox signalling. Biochem J. 2009 Dec 23;425(2):313-25. doi: 10.1042/BJ20091541 Go to original source... Go to PubMed...
  19. Wonsey DR, Zeller KI, Dang CV. The c-Myc target gene PRDX3 is required for mitochondrial homeostasis and neoplastic transformation. Proc Natl Acad Sci U S A. 2002 May 14;99(10):6649-54. doi: 10.1073/pnas.102523299 Go to original source... Go to PubMed...
  20. Coomarasamy A, Devall AJ, Brosens JJ, et al. Micronized vaginal progesterone to prevent miscarriage: a critical evaluation of randomized evidence. Am J Obstet Gynecol. 2020 Aug;223(2):167-176. doi: 10.1016/j.ajog.2019.12.006 Go to original source... Go to PubMed...
  21. Hernįndez-Rabaza V, López-Pedrajas R, Almansa I. Progesterone, Lipoic Acid, and Sulforaphane as Promising Antioxidants for Retinal Diseases: A Review. Antioxidants. 2019 Mar 2;8(3):53. doi: 10.3390/antiox8030053 Go to original source... Go to PubMed...
  22. Balasubramanian A1, Birundha S. Estimation of Glutathione Level in Second Trimester of Pregnancy without Complications; Sch Int J Biochem. Sep 2019; 2(9): 237-239. Go to original source...
  23. Kennedy L, Sandhu JK, Harper ME, et al. Role of Glutathione in Cancer: From Mechanisms to Therapies. Biomolecules. 2020 Oct 9;10(10):1429. doi: 10.3390/biom10101429 Go to original source... Go to PubMed...
  24. Bachhawat AK, Yadav S. The glutathione cycle: Glutathione metabolism beyond the γ-glutamyl cycle. IUBMB Life. 2018 Jul;70(7):585-592. doi: 10.1002/iub.1756 Go to original source... Go to PubMed...
  25. Wang Q, Wen X, Kong J. Recent Progress on Uric Acid Detection: A Review. Critical Reviews in Analytical Chemistry. 2020;50(4):359-375. doi: 10.1080/10408347.2019.1637711 Go to original source... Go to PubMed...
  26. Vignini A, Nanetti L, Moroni C, et al. Modifications of platelet from Alzheimer disease patients: a possible relation between membrane properties and NO metabolites. Neurobiol Aging. 2007 Jul;28(7):987-994. doi: 10.1016/j.neurobiolaging.2006.05.010 Go to original source... Go to PubMed...
  27. Al-Kuraishy HM, Al-Kuraishi AH, Al-Windy S, et al. Toxoplasmosis and risk of endothelial dysfunction: Role of oxidative stress and pro-inflammatory mediators. Archives of Clinical Infectious Diseases. 2019 Dec 31;14(6): e95563. Go to original source...
  28. Ra¹ię I, Ra¹ię A, Ak¹amija G, et al. The relationship between serum level of malondialdehyde and progression of colorectal cancer. Acta Clinica Croatica. 2018 Sep 1;57(3.):411-416. Go to original source... Go to PubMed...
  29. Janion K, Szczepańska E, Nowakowska-Zajdel E, et al. Selected Oxidative Stress Markers in Colorectal Cancer Patients in Relation to Primary Tumor Location-A Preliminary Research. Medicina (Kaunas). 2020 Jan 21;56(2):47. doi: 10.3390/medicina56020047 Go to original source... Go to PubMed...
  30. Samir D, Dalal D, Noura A. Study of oxidative stress during pregnancy. Global Journal of Pharmacy & Pharmaceutical Sciences. 2018;4(4):5. DOI: 10.19080/GJPPS.2018.04.555646 Go to original source...
  31. Sedlak J, Lindsay RH. Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent. Anal Biochem. 1968 Oct 24;25(1):192-205. doi: 10.1016/0003-2697(68)90092-4 Go to original source... Go to PubMed...
  32. Bahram D and Trinder P. The Estimation of Uric Acid. Analyst. 1972; 97: 142-145. Go to original source...
  33. Vanuffelen BE, Van Der Zee J, De Koster BM, et al. Intracellular but not extracellular conversion of nitroxyl anion into nitric oxide leads to stimulation of human neutrophil migration. Biochemical Journal. 1998 Mar 1;330 (2):719-722. doi: 10.1042/bj3300719 Go to original source... Go to PubMed...
  34. Muslih RK, Al-Nimer MS, Al-Zamely OY. The level of malondialdehyde after activation with H2O2 and CuSO4 and inhibition by deferoxamine and Molsidomine in the serum of patient with acute myocardial infraction. Nat. J. chem. 2002; 5:139-148.
  35. Armitage P, Berry G, Matthews JN. Statistical methods in medical research. John Wiley & Sons; 2008 Apr 15.
  36. Roumandeh N, Saremi A, Pooladi A, et al. Evaluation of serum Peroxiredoxin3 and Peroxiredoxin4 auto antibodies in recurrent spontaneous abortion patients. Sarem Journal of Medical research. 2018 Oct 10;3(3):159-163. Go to original source...
  37. Roumandeh N, Saremi A, Pooladi A, et al. Comparison of Serum Anti-Peroxiredoxin 3 and Anti- Peroxiredoxin 4 Auto Antibodies in the Patients with a Historyof Recurrent Spontaneous Abortion and Healthy Women. Sarem Journal of Reproductive Medicine. 2018; 2(4):159-163. Go to original source...
  38. Gharesi-Fard B, Jafarzadeh L, Ghaderi-shabankareh F, et al. Presence of autoantibody against two placental proteins, peroxiredoxin 3 and peroxiredoxin 4, in sera of recurrent pregnancy loss patients. American Journal of Reproductive Immunology. 2013 Mar;69(3):248-255. doi: 10.1111/aji.12042 Go to original source... Go to PubMed...
  39. Liu AX, Jin F, Zhang WW, et al. Proteomic analysis on the alteration of protein expression in the placental villous tissue of early pregnancy loss. Biol Reprod. 2006 Sep;75(3):414-420. doi: 10.1095/biolreprod.105.049379 Go to original source... Go to PubMed...
  40. Tanaka M, Miyazaki T, Tanigaki S, et al. Participation of reactive oxygen species in PGF2alpha-induced apoptosis in rat luteal cells. Journal of Reproduction and Fertility. 2000 Nov;120(2):239-245. doi: 10.1530/jrf.0.1200239 Go to original source... Go to PubMed...
  41. Celi P. The role of oxidative stress in small ruminants' health and production. Revista Brasileira de Zootecnia. 2010;39(Suppl.spe):348-363. DOI: 10.1590/S1516-35982010001300038 Go to original source...
  42. Hayashi K, Miyamoto A, Konari A, et al. Effect of local interaction of reactive oxygen species with prostaglandin F2α on the release of progesterone in ovine corpora lutea in vivo. Theriogenology. 2003 Mar;59(5-6):1335-44. doi: 10.1016/s0093-691x(02)01173-1 Go to original source... Go to PubMed...
  43. Sugino N. Roles of reactive oxygen species in the corpus luteum. Animal Science Journal. 2006;77:556-565. DOI: 10.1111/j.1740-0929.2006.00386.x Go to original source...
  44. Anfal D, Samir D. Study of fluoride-induced haematological alterations and liver oxidative stress in rats. World journal of pharmacy and pharmaceutical sciences. 2017 Mar 6;6(5):211-21. Go to original source...
  45. Dinēer Y, Alademir Z, Ilkova H, et al. Susceptibility of glutatione and glutathione-related antioxidant activity to hydrogen peroxide in patients with type 2 diabetes: effect of glycemic control. Clin Biochem. 2002 Jun;35(4):297-301. doi: 10.1016/s0009-9120(02)00317-x Go to original source... Go to PubMed...
  46. Ogueh O, Clough A, Hancock M, et al. A longitudinal study of the control of renal and uterine hemodynamic changes of pregnancy. Hypertens Pregnancy. 2011;30(3):243-59. doi: 10.3109/10641955.2010.484079 Go to original source... Go to PubMed...
  47. AL-Hamdani A H. Physiological Effect of Pregnancy on Some Renal Function Tests. Frontline Medical Sciences and Pharmaceutical Journal. 2022; Volume 02 Issue 05:5-15. Doi.10.37547/medical-fmspj-02-05-02
  48. Vazquez-Rodriguez JG. Role of serum uric acid in pre-eclampsia. Gynecol& Obstet Mex, 2011;79;292-297.
  49. AL-Hamdani IH. Measurement of Serum Uric Acid, Urea and Creatinine in Pregnant Women. The Medical Journal of Tikrit University. 2006 Volume 2, Issue 122: Pages 31-35.
  50. Raffaelli F, Nanetti L, Vignini A, et al. Nitric oxide platelet production in spontaneous miscarriage in the first trimester. Fertil Steril. 2010 Apr;93(6):1976-82. doi: 10.1016/j.fertnstert.2008.12.060 Go to original source... Go to PubMed...
  51. Issa AM, Hassan BG, Gatea AK. Relationship of Nitric Oxide and Malondialdehyde to Miscarriage. Medical Journal of Babylon. 2012;9(4):777-785.
  52. Torkzahrani S, Ataei1 PJ, Hedayati M, et al. Oxidative Stress Markers in Early Pregnancy Loss: A Case-Control Study. International Journal of Women's Health and Reproduction Sciences. 2019 Jan; Vol.7, No.1:61-66. Go to original source...
  53. Manila HD, Amir A, Anggraini ML, et al. The Differences in Malondialdehyde Levels Between Normal Pregnancy and Abortus. Advances in Health Sciences Research. 2021;Volume 39: 269-271.