MMSL 2023, 92(3):208-216 | DOI: 10.31482/mmsl.2022.042

EVALUATION OF ALFAXALONE'S ANTI-INFLAMMATORY AND ANTINOCICEPTIVE EFFECTS IN BROILER CHICKSOriginal article

Amjed Najem Alatroshi1, Ahmed Salah Naser ORCID...2*
1 Department of Physiology, Biochemistry and Pharmacology, Faculty of Veterinary Medicine, University of Mosul, Mosul, Iraq, amjadalatrushi@gmail.com
2 Department of Physiology, Biochemistry and Pharmacology, Faculty of Veterinary Medicine, University of Mosul, Mosul,Iraq, ahmadphd0@gmail.com

Background: Progesterone is the source of the steroid general anesthetic Alfaxalone, which became commercially available in the 1970s when combined  with alfadolone, another steroid anesthetic.

Objective: the goal of the present  research was to discover and assess the sub-anesthetic effects of Alfaxalone: the antinociceptive and anti-inflammatory properties in broiler chicks.

Methods: One hundred and three Ross broiler chicks (80-100 g, 7-8 days old) were used. The electric stimulation test, hot water test (HWT), and formalin test were performed on broiler chicks to assess the antinociceptive and anti-inflammatory properties of Alfaxalone. The up-and-down method that described by Dixon was used to determine the median effective antinociceptive dose.

Results: The median effective antinociceptive doses (ED50s) of Alfaxalone by  electric stimulation test and hot water test were 0.94 and 0.65 mg/kg intraperitoneally, respectively. Alfaxalone, at doses of 1, 2, and 4 mg/kg intraperitoneally, induced an antinociceptive effect in the electric stimulation test and HWT as well as antinociceptive and anti-inflammatory effects during the  formalin test in the chicks. The antinociceptive effect was displayed 15 min after treatment and the maximum effect was observed 30 min after injection.

Conclusion: The results showed that Alfaxalone had antinociceptive and anti-inflammatory effects, with a stronger antinociceptive effect in the electric stimulation test than in the thermal stimulation test, which was illustrated by  shorter antinociceptive time.

Keywords: broiler chicks; Alfaxalone; electrical stimulation; hot water test; anti-inflammatory

Received: July 17, 2022; Revised: August 25, 2022; Accepted: September 13, 2022; Prepublished online: January 16, 2023; Published: September 1, 2023  Show citation

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Najem Alatroshi, A., & Naser, A.S. (2023). EVALUATION OF ALFAXALONE'S ANTI-INFLAMMATORY AND ANTINOCICEPTIVE EFFECTS IN BROILER CHICKS. MMSL, 92(3), 208-216. doi: 10.31482/mmsl.2022.042
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