MMSL 2023, 92(4):348-355 | DOI: 10.31482/mmsl.2022.054

EFFECT OF MONTELUKAST ON HEALING OF INDUCED ORAL ULCER IN RATSOriginal article

Mohammed I. Majeed1*, Jawnaa K. Mammdoh ORCID...2, Luma I. Al-Allaf ORCID...3
1 Nineveh Health directorate, Mosul, Iraq
2 Department of Basic Sciences, College of Dentistry, University of Mosul, Mosul, Iraq
3 Department of Anatomy, College of Medicine, University of Mosul, Mosul, Iraq

Background: Oral ulcers are among the common recurrent oral diseases which seek medical help; the underlying pathology is yet obscure, making medical intervention difficult.

Aims: The purpose of this study was to investigate if montelukast could help mouth ulcer model in Albino rats.

Methods: The rats were divided into two groups of 24, the control group of 12 rats with induced ulcer and no therapy, and the treatment group with induced ulcer and treated orally with Montelukast 20 mg/kg. Each group had six rats sacrificed after 3 and 7 days of treatment. The lingual ulcer was produced with cotton soaked in 70% acetic acid solution and rubbed against the tongue for 2 minutes. Discolouration of tongue tissue has been noticed. Animals in all groups were weighed on days 1, 3, and 7 following mucosal ulcerations were confirmed.

Results: On days 1, 3, and 7, the body weight of the treatment group rats improved as compared to the control group. On days 3 and 7, the tongue histological section of the control group revealed a wide gap of the site of ulcer in the lingual mucosa, inflammatory exudate, and severe infiltration of inflammatory cells (score 3) without re-epithelialization (score 0). Lingual sections of MTK treated group after ulcer induction on day 3 showed the same lesions as the control group whereas after 7 days demonstrated improvement in inflammatory indicators as inflammatory exudate and infiltration of inflammatory cells (score 1), formation of granulation tissue composed of fibrous tissue and angiogenesis and Re-epithelialization (score 1) comparing with the control group.

Conclusion: The present study found Montelukast's anti-inflammatory potential characteristics to treat generated lingual ulcers in rats.

Keywords: Montelukast; Healing; Oral Ulcer; Rats

Received: July 20, 2022; Revised: October 25, 2022; Accepted: December 7, 2022; Prepublished online: February 6, 2023; Published: December 1, 2023  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Majeed, M.I., Mammdoh, J.K., & Al-Allaf, L.I. (2023). EFFECT OF MONTELUKAST ON HEALING OF INDUCED ORAL ULCER IN RATS. MMSL, 92(4), 348-355. doi: 10.31482/mmsl.2022.054
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Rezazadeh F, Nazhvani AD, Abolmaali SS, et al. Efficacy of silver nanoparticle gel on healing of traumatic oral ulcers compared with triamcinolone oral paste: An experimental study on rats. Dental research journal. 2021;18. Go to original source... Go to PubMed...
    2. Wu W, Ruan J, Li D, et al. Effect of dexamethasone on levels of inflammatory factors and EGF mRNA in rabbits suffering from oral ulcers. Tropical Journal of Pharmaceutical Research. 2021;20(2):351-357. https://doi.org/10.4314/tjpr.v20i2.19 Go to original source...
    3. Idrus E, Hartanti PD, Suniarti DF, et al. An experimental model of chemically-induced ulceration of the buccal mucosa of Mus musculus. Makara Journal of Health Research. 2019;23(3):10. https://doi.org/10.7454/msk.v23i3.1158 Go to original source...
    4. Al-Abdaly YZ, Saeed MG, Al-Hashemi HM. Effect of methotrexate and aspirin interaction and its relationship to oxidative stress in rats. Iraqi J Vet Sci. 2021;35(1):151-156. https://doi.org/10.33899/ijvs.2020.126490.1335 Go to original source...
    5. Nazir MA, Almas K. Awareness about the effects of tobacco consumption on oral health and the possibility of smoking behavior among male Saudi schoolchildren. Eur J Dent. 2017;11(1):29-35. https://doi.org/10.4103/ejd.ejd_300_16 Go to original source... Go to PubMed...
    6. Mahattanadul S, Mustafa MW, Kuadkaew S, et al. Oral ulcer healing and anti-candida efficacy of an alcohol-free chitosan-curcumin mouthwash. Eur Rev Med Pharmacol Sci. 2018 Oct 1;22(20):7020-7023. https://doi.org/10.26355/eurrev_201810_16173 Go to original source... Go to PubMed...
    7. Chen P, Yao H, Su W, et al. Pharmacodynamic and Metabolomics Studies on the Effect of Kouyanqing Granule in the Treatment of Phenol-Induced Oral Ulcer Worsened by Sleep Deprivation. Frontiers in pharmacology. 2020:824. https://doi.org/10.3389/fphar.2020.00824 Go to original source... Go to PubMed...
    8. Wen SD, Sans-Serramitjana E, Santander JF, et al. Effects of natural extracts in the treatment of oral ulcers: A systematic review of evidence from experimental studies in animals. Immediate and long-term microshear bond strength of resin-based cements to core build-up materials. J Clin Exp Dent. 2021;13(10):e1038-1048. https://doi.org/10.4317/jced.58567. Go to original source... Go to PubMed...
    9. Abdel-Raheem IT, Khedr NF. Renoprotective effects of montelukast, a cysteinyl leukotriene receptor antagonist, against methotrexate-induced kidney damage in rats. N-S Arch Pharmacol. 2014;387:341-353. https://doi.org/10.1007/s00210-013-0949-x Go to original source... Go to PubMed...
    10. El-Sisi AE, Sokar SS, Salem TA, et al. Role of cysteinyl leukotriene receptor-1 antagonists in treatment of experimentally induced mammary tumor: does montelukast modulate antitumor and immunosuppressant effects of doxorubicin? Toxicol Ind Health. 2015;31:1024-1036. https://doi.org/10.1177/07482337134858 Go to original source... Go to PubMed...
    11. Matsuyama M, Hayama T, Funao K, et al. Overexpression of cysteinyl LT1 receptor in prostate cancer and CysLT1R antagonist inhibits prostate cancer cell growth through apoptosis. Oncol Rep. 2007;18:99-104. https://doi.org/10.3892/or.18.1.99 Go to original source... Go to PubMed...
    12. Theron AJ, Steel HC, Tintinger GR, et al. (2014) Cysteinyl leukotriene receptor-1 antagonists as modulators of innate immune cell function. J Immunol Res 608930. https://doi.org/10.1155 /2014/608930 Go to original source... Go to PubMed...
    13. Kuru S, Kismet K, Barlas AM, et al. The effect of montelukast on liver damage in an experimental obstructive jaundice model. Viszeralmedizin. 2015;31:131- 138. https://doi.org/10.1159/000375434 Go to original source... Go to PubMed...
    14. Wu S, Zhu X, Jin Z, et al. The protective role of montelukast against intestinal ischemia-reperfusion injury in rats. 2015;Sci Rep 5:15787. https://doi.org/10.1038 /srep15787 Go to original source... Go to PubMed...
    15. Tintinger GR, Feldman C, Theron AJ, et al. Montelukast: more than a cysteinyl leukotriene receptor antagonist?. TheScientificWorldJournal. 2010 Dec 14;10:2403-2413. https://doi.org/10.1100/tsw.2010.229 Go to original source... Go to PubMed...
    16. Abdelhady SA, Ali MA, Al-Shafie TA, et al. Montelukast potentiates the antiinflammatory effect of NSAIDs in the rat paw formalin model and simultaneously minimizes the risk of gastric damage. Inflammation Research. 2021 Sep;70(9):981-992. https://doi.org/10.1007/s00011-021-01492-9 Go to original source... Go to PubMed...
    17. Takemura M, Niimi A, Matsumoto H, et al. Clinical, physiological and anti-inflammatory effect of montelukast in patients with cough variant asthma. Respiration. 2012;83(4):308-315. https://doi.org/10.1159/000332835 Go to original source... Go to PubMed...
    18. Said MM, Bosland MC. The anti-inflammatory effect of montelukast, a cysteinyl leukotriene receptor-1 antagonist, against estradiol-induced nonbacterial inflammation in the rat prostate. Naunyn-Schmiedeberg's archives of pharmacology. 2017 Feb;390(2):197-205. https://doi.org/10.1007/s00210-016-1325-4 Go to original source... Go to PubMed...
    19. Halici M, Odabasoglu F, Suleyman H, et al. Effects of water extract of Usnea longissima on antioxidant enzyme activity and mucosal damage caused by indomethacin in rats. Phytomedicine. 2005 Sep 15;12(9):656-662. https://doi.org/10.1016/j.phymed.2004.06.021. Go to original source... Go to PubMed...
    20. Hayes JD, Pulford DJ. The glut athione S-transferase supergene family: regulation of GST and the contribution of the lsoenzymes to cancer chemoprotection and drug resistance part I. Critical reviews in biochemistry and molecular biology. 1995 Jan 1;30(6):445-520. https://doi.org/10.3109/10409239509083491. Go to original source... Go to PubMed...
    21. Idrus E, Pramatama IA, Suniarti DF, et al. Experimental Model of Thermally Induced-Tongue Ulcer in Mice. Journal of International Dental and Medical Research. 2019 Sep 1;12(3):929-934.
    22. Kwon YB, Kim HW, Roh DH, et al. Topical application of epidermal growth factor accelerates wound healing by myofibroblast proliferation and collagen synthesis in rat. Journal of veterinary science. 2006 Jun 1;7(2):105-109. https://doi.org/10.4142/jvs.2006.7.2.105. Go to original source... Go to PubMed...
    23. Mammdoh JK, Al-Mashhadanee FA, Al-Moula AD, et al. Effect of Topical Serratiopeptidase on Facial Wound Healing in Rabbit. 2020; 12(6): 284-290
    24. Clinical Trial. Effect of taking Montelukast tablets in mouth ulcers. CTRI/2017/11/010522.https://trialsearch.who.int/Trial2.aspx?TrialID=CTRI/2017/11/010522, 2017 | added to CENTRAL: 31 March 2019 | 2019 Issue 3. 2019;(3).
    25. ªener G, Kapucu C, Cetinel S, et al. Gastroprotective effect of leukotriene receptor blocker montelukast in alendronat-induced lesions of the rat gastric mucosa. Prostaglandins Leukotrienes and Essential Fatty Acids. 2005;72(1):1-11. https://doi.org/10.1016/j.plefa.2004.04.005 Go to original source... Go to PubMed...
    26. Femiano F, Buonaiuto C, Gombos F, et al. Pilot study on recurrent aphthous stomatitis (RAS): a randomized placebo-controlled trial for the comparative therapeutic effects of systemic prednisone and systemic montelukast in subjects unresponsive to topical therapy. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology. 2010 Mar 1;109(3):402-407. https://doi.org/10.1016/j.tripleo.2009.10.024 Go to original source... Go to PubMed...
    27. Aquino TM, Jamora MJ. Primary Idiopathic Complex Aphthosis: Diagnosis and Successful Treatment with Montelukast in a 44-Year-Old Filipino Female. Case Reports in Dermatology. 2020;12(1):12-18. https://doi.org/10.1159/000505475 Go to original source... Go to PubMed...
    28. Pu S, Liu Q, Li Y, et al. Montelukast prevents mice against acetaminophen-induced liver injury. Frontiers in pharmacology. 2019:1070. https://doi.org/10.3389/fphar.2019.01070 Go to original source... Go to PubMed...
    29. Soltanieh A, Avizeh R, et al. Antioxidant Effect of Montelukast on Acute Lung Injury Induced by Lipopolysaccharide in Dogs. Journal of Babol University of Medical Sciences. 2021 Mar 10;23(1):229-235.
    30. Elrashidy RA, Hasan RA. Modulation of autophagy and transient receptor potential vanilloid 4 channels by montelukast in a rat model of hemorrhagic cystitis. Life Sciences. 2021 Aug 1;278:119507. https://doi.org/10.1016/j.lfs.2021.119507. Go to original source... Go to PubMed...
    31. El-shafaei A, Abdelmaksoud R, Elshorbagy A, et al. Protective effect of melatonin versus montelukast in cisplatin-induced seminiferous tubule damage in rats. Andrologia. 2018 Nov;50(9):e13077. https://doi.org/10.1111/and.13077 Go to original source... Go to PubMed...
    32. Wafaa K. Abid, Alyaa I. Naser.The efficacy of a new paste formulation as an alternative therapeutic agent for traumatic ulcers.Journal of Taibah University Medical Sciences. 2021;16(5):724-732. https://doi.org/10.1016/j.jtumed.2021.05.005. Go to original source... Go to PubMed...
    33. Kolhe AM, Kale A. Evaluation of analgesic, anti-inflammatory, and antipyretic activity of leukotriene receptor antagonist-montelukast: An experimental study. National Journal of Physiology, Pharmacy and Pharmacology. 2017;7(1):32. https://doi.org/10.5455/njppp.2016.6.0616811072016. Go to original source...

    Return to the content