VZL 2007, 76(6):236-241

Characterization of Preparation of Equine Serum Butyrylcholinesterase in vitro and Its Blood Levels in Rats

Lucie Bartošová, Jiří Bajgar
Univerzita obrany, katedra toxikologie Fakulty vojenského zdravotnictví, Hradec Králové

U preparátu koňské butyrylcholinesterázy byla stanovena Michaelisova konstanta in vitro se substrátem butyrylthiocholin jodid pro dvě koncentrační rozmezí koncentrace substrátu potvrzující aktivaci enzymu substrátem (Km 59,7 μM a Kss 1,02 mM) a pI50 pro sarin (7,06 nM). Po i. p. podání laboratorním potkanům byly stanoveny aktivity cholinesteráz v krvi, které přetrvávají až do 72 hodin po aplikaci.

Keywords: Preparát; Koňská butyrylcholinesteráza; Charakterizace; Potkan

Michaelis constant in two concentration ranges of substrate (Km 59.7 μM and Kss 1.02 mM) evidencing activation of the enzyme by substrate and pI50 (7.06 nM) value for preparation of equine butyrylcholinesterase were determined in vitro. Activities of cholinesterases in the blood of rats were determined following i. p. administration lasting 72 hours after the administration.

Keywords: Preparation; Equine butyrylcholinesterase; Characterization; Rat

Received: February 1, 2007; Published: December 1, 2007  Show citation

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Bartošová, L., & Bajgar, J. (2007). Characterization of Preparation of Equine Serum Butyrylcholinesterase in vitro and Its Blood Levels in Rats. Vojenské Zdravotnické Listy, 76(6), 236-241
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