VZL 2009, 78(1):7-9
Monitoring of Antibodies Reaction against Francisella Tularensis Using the ELISA Method
- Oto Pavliš1, Miroslav Pohanka
ORCID...1,2 - 1 Ústřední vojenský zdravotní ústav, Centrum biologické ochrany, Těchonín
- 2 Univerzita obrany, Fakulta vojenského zdravotnictví, Centrum pokročilých studií, Hradec Králové
Mikrorganismus Francisella tularensis vyvolává závažné onemocnění lidí a zvířat známé pod označením tularémie. Sledování protilátkové odpovědi na infekci způsobenou tímto mikroorganismem, resp. kmenem s označením LVS (live vaccine strain) bylo provedeno sérologickým vyšetřením vzorků krve získaných od imunizovaných myší inbredního kmene BALB/c v intervalu 1 až 21 dní. Pomocí metody ELISA byly stanoveny a vyhodnoceny titry celkových protilátek a dále izotypových tříd IgM a IgG. V práci se diskutuje o možnosti použití metody ELISA pro diagnostiku tularémie v ranných fázích.
Keywords: Francisella tularensis; Tularémie; ELISA; BALB, c
Microorganism Francisella tularensis is the etiological agent of tularemia, a serious disease of humans and animals. Serological diagnosis of antibodies titer in mice models was performed by the indirect ELISA. Inbred mice BALB/c were divided into ten intervals and all of them (without a control group) were inoculated with Francisella tularensis LVS. Obtained blood samples or more precisely sera were collected from 1 to 21 days. Antibodies titers of total immunoglobulins and isotypes IgG and IgM were assessed and finally the progress of antibodies against F. tularensis LVS was characterized. Applicability of ELISA for tularemia diagnosis is discussed.
Keywords: Francisella tularensis; Tularemia; ELISA; BALB/c
Received: March 6, 2008; Published: March 1, 2009 Show citation
References
- CULKIN, SJ. - RHINEHART-JONES, T. - ELKINS, KL. A novel role for B cells in early protective immunity to an intracellular pathogen, Francisella tularensis strain LVS. J. Immunol., 1997, vol. 158, p. 3277-3284.
Go to PubMed... - DREISBACH, VC. - COWLEY, S. - ELKINS KL. Purified lipopolysaccharide from Francisella tularensis live vaccine strain (LVS) induces protective immunity against LVS infection that requires B cells and gamma interferon. Infect. Immun., 2000, vol. 68, p. 1988-1996.
Go to original source... Go to PubMed... - EIGELSBACH, HT., et al. Live tularemia vaccine. I. Host-parasite relationship in monkeys vaccinated intracutaneously or aerogenically. J. Bacteriol., 1962, vol. 84, p. 1020-1027. Go to original source... Go to PubMed...
- ELLIS, J., et al. Tularemia. Clin. Microbiol. Rev., 2002, vol. 1, p. 631-646. Go to original source... Go to PubMed...
- GURYČOVÁ, D. First isolation of Francisella tularensis subsp. tularensis in Europe. Eur. J. Epidem., 1998, vol. 14, p. 797-802.
- HUBÁLEK Z. - HALOUZKA J. Mosquitoes (Diptera: Culicidae), in contrast to ticks (Acari: Ixodidae), do not carry Francisella tularensis in a natural focus of tularemia in the Czech Republic. J. Med. Entomol., Nov 1997, vol. 34, no. 6, p. 660-663. Go to original source... Go to PubMed...
- LOPEZ, MC., et al. Early activation of NK cells after lung infection with the intracellular bacterium, Francisella tularensis LVS. Cell Immunol., 2004, vol. 232, p. 75-85. Go to original source... Go to PubMed...
- McCOY, GW. Studies upon plague in ground squirrels. II. A plague-like disease of rodents.: Publ. Health Bull. (Wash.), 1911, vol. 43, p. 53-71.
- McCOY, GW. - CHAPIN, CW. Further observation on a plague-like disease of rodents with a preliminary note on the causative agent: Bacterium tularense. J. Infect. Dis., 1912, vol. 10, p. 61-72. Go to original source...
- POHANKA, M. - PAVLIŠ, O. - SKLÁDAL, P. Diagnosis of tularemia using piezoelectric biosensor technology. Talanta, 2007, vol. 71, p. 981-985. Go to original source... Go to PubMed...
- POHANKA, M. - PAVLIŠ, O. - SKLÁDAL, P. Rapid characterization of monoclonal antibodies using the piezoelectric immunosenzor. Sensors, 2007, vol. 7, p. 341-353. Go to original source...