Vojenské Zdravotnické Listy, 2009 (vol. 78), issue 1

Colour Supplement

VZL 2009, 78(1):I-IV

Urine Analysis

Miloš Tichý, Václav Monhart

VZL 2009, 78(1):1-6

Early determination of diagnosis and initiation of therapy is very significant in kidney disorder. In submitted publication we give a brief survey of methods, which clinical biochemistry offers for examination of urine nowadays. The publication gives a short review of the history of urine analysis and deals with new methods for determination of glomerular filtration in details.

Monitoring of Antibodies Reaction against Francisella Tularensis Using the ELISA Method

Oto Pavliš, Miroslav Pohanka

VZL 2009, 78(1):7-9

Microorganism Francisella tularensis is the etiological agent of tularemia, a serious disease of humans and animals. Serological diagnosis of antibodies titer in mice models was performed by the indirect ELISA. Inbred mice BALB/c were divided into ten intervals and all of them (without a control group) were inoculated with Francisella tularensis LVS. Obtained blood samples or more precisely sera were collected from 1 to 21 days. Antibodies titers of total immunoglobulins and isotypes IgG and IgM were assessed and finally the progress of antibodies against F. tularensis LVS was characterized. Applicability of ELISA for tularemia diagnosis is discussed.

Proteome Analysis of Bacterium Borrelia Afzelii

Věra Neubauerová, Aleš Macela, Lenka Hernychová, Juraj Lenčo, Martin Brychta, Jana Havlasová, Jan Kopecký, Jitka Pechová

VZL 2009, 78(1):10-14

Borrelia afzelii is one of potential etiological agents of Lyme disease in the Czech Republic. An important source of information about this microoganism is proteome analysis. It allows to understand pathogenesis of Lyme disease. The proteins were identified by 2-dimensional electrophoresis and mass spectrometry (MALDI-TOF). Overall 34 protein spots of Borrelia afzelii (strain CB43) were chosen for identification. This process was done successfully in case of 23 proteins. This number was represented by 11 different proteins. We identified some variants of OspC protein, several enzymes, protein of chemotaxis CheY and Membrane associated...

Artery Ballon Angioplasty and Depression: Findings of Prospective, Longitudinal Study

Ladislav Slováček, Birgita Slováčková, Iva Slánská, Ladislav Jebavý, Zdeněk Hrstka, Vladimír Pavlík

VZL 2009, 78(1):15-18

Background: Peripheral arterial occlusive disease (PAOD) as a chronic disease is associated with physical, psychological and social distress for elderly patients and their families. Aims: The study has two main aims: 1. To evaluate the occurrence of depression in patients with PAOD, 2. To evaluate the effect of percutaneous trasluminal balloon angioplasty on occurence of depression. Material and methods: The study was local, prospective and longitudinal. Data were obtained during 2006. The total number of subjects with PAOD was 42 (28 males, 14 females). The average age of all subjects with PAOD was 65.4 years (aged 45-79 years). The evaluation of...

Effect of Triazine Based Pesticide on Fish

Vratislav Malý, Josef Velíšek

VZL 2009, 78(1):19-23

Effect of triazine based pesticide (Sencor 70 WG - active substance metribuzine) was assessed via biochemical and hematological blood profile determination of experimental fish. We used a common carp (Cyprinus carpio L.) as an experimental fish. Before rating the influence of metribuzine on biochemical and hematological blood profile we accomplished a preliminary test to determine a concentration range and after that a basic acute toxicity test to determine 96hLC50 value. Samples of common carp fingerlings were used for performing the tests. After 96hLC50 determination two groups of experimental fish (aged 1-2 years) were under this concentration and...

New Chemical Terrorism Weapon - Osmium Tetroxide

Zdeněk Hon, Jiří Patočka, Vladimír Pitschmann

VZL 2009, 78(1):24-27

A highly toxic chemicals represent possible means of terrorism. Such less usual chemical is osmium teroxide (OsO₄), a powerful oxidizer which effects mainly the skin and mucous membranes. Already in a small amount it causes irritation of eyes, nose, throat and skin. OsO₄ is an acute poison. It can cause of causing serious health damage or death very quickly. It is toxic if it is inhaled, swallowed, or absorbed through the skin. Long-term exposure of OsO₄ can cause permanent damage of eyes, including blindness. Although it is unsuitable for a large-scale terrorist attack, mainly due to its scarcity, it could be used...

Czech Post at the ISAF Headquarters, Afghanistan

Vladimír Pavlík

VZL 2009, 78(1):28-31

The autor describes Czech medical position at the HQ ISAF in Kabul. At the same time the article describes medical support to ISAF in Afghanistan by Medical treatment facilities and also medical evacuation by rotary wings and fixed wings / MEDEVAC / in the battle field under Medical operations office control.