VZL 2009, 78(1):10-14

Proteome Analysis of Bacterium Borrelia Afzelii

Věra Neubauerová1, Aleš Macela ORCID...2, Lenka Hernychová2, Juraj Lenčo2, Martin Brychta2, Jana Havlasová2, Jan Kopecký3, Jitka Pechová3
1 Ústřední vojenský zdravotní ústav, Praha
2 Univerzita obrany, Fakulta vojenského zdravotnictví, Ústav molekulární patologie, Hradec Králové
3 Akademie věd ČR, Parazitologický ústav, České Budějovice

Borrelia afzelii je jedním z možných původců lymské boreliózy v České republice. Důležitým zdrojem informací o tomto mikroorganismu je v současnosti proteomová analýza, která svými výsledky umožňuje pochopit vznik a průběh tohoto onemocnění. Proteiny byly identifikovány pomocí dvojrozměrové elektroforézy a hmotnostní spektrometrie (MALDI-TOF). Celkem bylo pro identifikační proces vybráno 34 proteinových spot Borrelia afzelii (kmen CB43). Identifikace byla úspěšná u 23 proteinů. Tento počet představoval 11 různých proteinů. Identifikováno bylo několik variant OspC proteinu, dále několik proteinů s enzymatickou funkcí, protein zapojený do procesu chemotaxe CheY a prekurzor membránového proteinu P66.

Keywords: Borrelia afzelii; Proteomová analýza; 2-DE; MALDI-TOF MS

Borrelia afzelii is one of potential etiological agents of Lyme disease in the Czech Republic. An important source of information about this microoganism is proteome analysis. It allows to understand pathogenesis of Lyme disease. The proteins were identified by 2-dimensional electrophoresis and mass spectrometry (MALDI-TOF). Overall 34 protein spots of Borrelia afzelii (strain CB43) were chosen for identification. This process was done successfully in case of 23 proteins. This number was represented by 11 different proteins. We identified some variants of OspC protein, several enzymes, protein of chemotaxis CheY and Membrane associated protein p66 precursor.

Keywords: Borrelia afzelii; Proteome analysis; 2-DE; MALDI-TOF MS

Received: October 20, 2008; Published: March 1, 2009  Show citation

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Neubauerová, V., Macela, A., Hernychová, L., Lenčo, J., Brychta, M., Havlasová, J., Kopecký, J., & Pechová, J. (2009). Proteome Analysis of Bacterium Borrelia Afzelii. Vojenské Zdravotnické Listy, 78(1), 10-14
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