VZL 2010, 79(2):54-59

"Sweet" World of Bacteria: Methodical Approaches Used in Bacterial Glycoproteins Analysis

Lucie Balonová1,2, Lenka Hernychová1, Zuzana Bílková2, Jiří Stulík ORCID...1
1 Univerzita obrany, Fakulta vojenského zdravotnictví, Ústav molekulární patologie, Hradec Králové
2 Univerzita Pardubice, Fakulta chemicko-technologická, katedra analytické chemie a biologických a biochemických věd, Pardubice

Glykosylace proteinů hraje významnou úlohu v patogenitě některých bakterií. V předloženém příspěvku uvádíme jednak přehled současných analytických metod, které se využívají v analýze glykosylací eukaryotických proteinů, a dále pak metody s experimentálně ověřenými modifikacemi, které mohou být aplikovány při studiu prokaryotických glykoproteinů.

Keywords: Glykosylace; Glykan; Glykoprotein; Chromatografie; Hmotnostní spektrometrie

Protein glycosylation plays an important role in the pathogenicity of certain bacteria. In this article, we provide an overview of up-to-date analytical methods, which are utilized in the analysis of protein glycosylation of eukaryotic origin. The methods with experimentally verified modifications can be applied in a study of prokaryotic glycoproteins as well.

Keywords: Glycosylation; Glycan; Glycoprotein; Chromatography; Mass spectrometry

Received: August 17, 2009; Published: June 1, 2010  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Balonová, L., Hernychová, L., Bílková, Z., & Stulík, J. (2010). "Sweet" World of Bacteria: Methodical Approaches Used in Bacterial Glycoproteins Analysis. Vojenské Zdravotnické Listy, 79(2), 54-59
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. ALLEY, WR., Jr. - MECHREF, Y. - NOVOTNY, MV. Use of activated graphitized carbon chips for liquid chromatography/mass spectrometric and tandem mass spectrometric analysis of tryptic glycopeptides. Rapid. Commun. Mass. Spectrom., 2009, vol. 23, no. 4, p. 495-505. Go to original source... Go to PubMed...
    2. ARORA, SK., et al. Role of motility and flagellin glycosylation in the pathogenesis of Pseudomonas aeruginosa burn wound infections. Infect. Immun., 2005, vol. 73, no. 7, p. 4395-4398. Go to original source... Go to PubMed...
    3. BALONOVA, L. - HERNYCHOVA, L. - BILKOVA, Z. Bioanalytical tools for the discovery of eukaryotic glycoproteins applied to the analysis of bacterial glycoproteins. Expert. Rev. Proteomics, 2009, vol. 6, no. 1, p. 75-85. Go to original source... Go to PubMed...
    4. BAUSE, E. Structural requirements of N-glycosylation of proteins. Studies with proline peptides as conformational probes. Biochem. J., 1983, vol. 209, no. 2, p. 331-336. Go to original source... Go to PubMed...
    5. BOBBITT, JM. Periodate oxidation of carbohydrates. Adv. Carbohydr. Chem., 1956, vol. 48, no. 11, p. 1-41. Go to original source... Go to PubMed...
    6. CARLSON, DM. Structures and immunochemical properties of oligosaccharides isolated from pig submaxillary mucins. J. Biol. Chem., 1968, vol. 243, no. 3, p. 616-626. Go to original source... Go to PubMed...
    7. CIUCANU, I. - KEREK, F. A simple and rapid method for the permethylation of carbohydrates. Carbohydr. Res., 1984, vol. 131, p. 209-217. Go to original source...
    8. CLAVEROL, S., et al. Characterization of protein variants and post-translational modifications: ESI-MSn analyses of intact proteins eluted from polyacrylamide gels. Mol. Cell. Proteomics, 2003, vol. 2, no. 8, p. 483-493. Go to original source... Go to PubMed...
    9. CUMMINGS, RD. Use of lectins in analysis of glycoconjugates. Methods Enzymol., 1994, vol. 230, p. 66-86. Go to original source... Go to PubMed...
    10. DUFFIN, KL., et al. Characterization of N-linked oligosaccharides by electrospray and tandem mass spectrometry. Anal. Chem., 1992, vol. 64, no. 13, p. 1440-1448. Go to original source... Go to PubMed...
    11. GAVEL, Y. - HEIJNE, G., von. Sequence differences between glycosylated and non-glycosylated Asn-X-Thr/Ser acceptor sites: implications for protein engineering. Protein. Eng., 1990, vol. 3, no. 5, p. 433-442. Go to original source... Go to PubMed...
    12. GOETZ, JA., et al. Glycomic profiling of invasive and non-invasive breast cancer cells. Glycoconj. J., 2009, vol. 26, no. 2, p. 117-131. Go to original source... Go to PubMed...
    13. HART, C., et al. Detection of glycoproteins in polyacrylamide gels and on electroblots using Pro-Q Emerald 488 dye, a fluorescent periodate Schiff-base stain. Electrophoresis, 2003, vol. 24, no. 4, p. 588-598. Go to original source... Go to PubMed...
    14. HUANG, Y. - MECHREF, Y. - NOVOTNY, MV. Microscale nonreductive release of O-linked glycans for subsequent analysis through MALDI mass spectrometry and capillary electrophoresis. Anal. Chem., 2001, vol. 73, no. 24, p. 6063-6069. Go to original source... Go to PubMed...
    15. KARLYSHEV, AV., et al. The Campylobacter jejuni general glycosylation system is important for attachment to human epithelial cells and in the colonization of chicks. Microbiology, 2004, vol. 150, Pt 6, p. 1957-1964. Go to original source... Go to PubMed...
    16. KOIZUMI, K. High-performance liquid chromatographic separation of carbohydrates on graphitized carbon columns. J. Chromatogr. A., 1996, vol. 720, no. 1/2, p. 119-126. Go to original source... Go to PubMed...
    17. KOWARIK, M., et al. Definition of the bacterial N-glycosylation site consensus sequence. Embo. J., 2006, vol. 25, no. 9, p. 1957-1966. Go to original source... Go to PubMed...
    18. KYSELOVA, Z., et al. Breast cancer diagnosis and prognosis through quantitative measurements of serum glycan profiles. Clin. Chem., 2008, vol. 54, no. 7, p. 1166-1175. Go to original source... Go to PubMed...
    19. LEE, JH., et al. Immobilization of aminophenylboronic acid on magnetic beads for the direct determination of glycoproteins by matrix assisted laser desorption ionization mass spectrometry. J. Am. Soc. Mass. Spectrom., 2005, vol. 16, no. 9, p. 1456-1460. Go to original source... Go to PubMed...
    20. MADERA, M., et al. High-sensitivity profiling of glycoproteins from human blood serum through multiple-lectin affinity chromatography and liquid chromatography/tandem mass spectrometry. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2007, vol. 845, no. 1, p. 121-137. Go to original source... Go to PubMed...
    21. MADERA, M. - MECHREF, Y. - NOVOTNY, MV. Combining lectin microcolumns with high-resolution separation techniques for enrichment of glycoproteins and glycopeptides. Anal. Chem., 2005, vol. 77, no. 13, p. 4081-4090. Go to original source... Go to PubMed...
    22. MAKI, M. - RENKONEN, R. Biosynthesis of 6-deoxyhexose glycans in bacteria. Glycobiology, 2004, vol. 14, no. 3, p. 1R-15R. Go to original source... Go to PubMed...
    23. MECHREF, Y. - NOVOTNY, MV. Glycomic analysis by capillary electrophoresis-mass spectrometry. Mass. Spectrom. Rev., 2009, vol. 28, no. 2, p. 207-222. Go to original source... Go to PubMed...
    24. MECHREF, Y. - NOVOTNY, MV. Structural investigations of glycoconjugates at high sensitivity. Chem. Rev., 2002, vol. 102, no. 2, p. 321-369. Go to original source... Go to PubMed...
    25. NINONUEVO, M., et al. Nanoliquid chromatography-mass spectrometry of oligosaccharides employing graphitized carbon chromatography on microchip with a high-accuracy mass analyzer. Electrophoresis, 2005, vol. 26, no. 19, p. 3641-3649. Go to original source... Go to PubMed...
    26. NITA-LAZAR, M., et al. The N-X-S/T consensus sequence is required but not sufficient for bacterial N-linked protein glycosylation. Glycobiology, 2005, vol. 15, no. 4, p. 361-367. Go to original source... Go to PubMed...
    27. O'SHANNESSY, DJ. Hydrazido-derivatized supports in affinity chromatography. J. Chromatogr., 1990, vol. 510, p. 13-21. Go to original source... Go to PubMed...
    28. PAPAC, DI. - WONG, A. - JONES, AJ. Analysis of acidic oligosaccharides and glycopeptides by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal. Chem., 1996, vol. 68, no. 18, p. 3215-3223. Go to original source... Go to PubMed...
    29. PATEL, T., et al. Use of hydrazine to release in intact and unreduced form both N- and O-linked oligosaccharides from glycoproteins. Biochemistry, 1993, vol. 32, no. 2, p. 679-693. Go to original source... Go to PubMed...
    30. SALZANO, AM. - CRESCENZI, M. Mass spectrometry for protein identification and the study of post translational modifications. Ann. Ist. Super. Sanita., 2005, vol. 41, no. 4, p. 443-450. Go to PubMed...
    31. STEINBERG, TH., et al. Rapid and simple single nanogram detection of glycoproteins in polyacrylamide gels and on electroblots. Proteomics, 2001, vol. 1, no. 7, p. 841-855. Go to original source... Go to PubMed...
    32. STERBA, J., et al. Flagellin and outer surface proteins from Borrelia burgdorferi are not glycosylated. J. Bacteriol., 2008, vol. 190, no. 7, p. 2619-2623. Go to original source... Go to PubMed...
    33. SZYMANSKI, CM. - BURR, DH. - GUERRY, P. Campylobacter protein glycosylation affects host cell interactions. Infect. Immun., 2002, vol. 70, no. 4, p. 2242-2244. Go to original source... Go to PubMed...
    34. SZYMANSKI, CM., et al. Evidence for a system of general protein glycosylation in Campylobacter jejuni. Mol. Microbiol., 1999, vol. 32, no. 5, p. 1022-1030. Go to original source... Go to PubMed...
    35. TARENTINO, AL. - GOMEZ, CM. - PLUMMER, TH. Jr. Deglycosylation of asparagine-linked glycans by peptide: N-glycosidase F. Biochemistry, 1985, vol. 24, no. 17, p. 4665-4671. Go to original source... Go to PubMed...
    36. UEDA, K., et al. Targeted serum glycoproteomics for the discovery of lung cancer-associated glycosylation disorders using lectin-coupled ProteinChip arrays. Proteomics, 2009, vol. 9, no. 8, p. 2182-2192. Go to original source... Go to PubMed...
    37. VISEUX, N. - DE HOFFMANN, E. - DOMON, B. Structural assignment of permethylated oligosaccharide subunits using sequential tandem mass spectrometry. Anal. Chem., 1998, vol. 70, no. 23, p. 4951-4959. Go to original source... Go to PubMed...
    38. WEISKOPF, AS. - VOUROS, P. - HARVEY, DJ. Characterization of oligosaccharide composition and structure by quadrupole ion trap mass spectrometry. Rapid. Commun. Mass. Spectrom., 1997, vol. 11, no. 14, p. 1493-1504. Go to original source... Go to PubMed...
    39. WUHRER, M., et al. Glycoproteomics based on tandem mass spectrometry of glycopeptides. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2007, vol. 849, no. 1/2, p. 115-128. Go to original source... Go to PubMed...
    40. YOUNG, NM., et al. Structure of the N-linked glycan present on multiple glycoproteins in the Gram-negative bacterium, Campylobacter jejuni. J. Biol. Chem., 2002, vol. 277, no. 45, p. 42530-42539. Go to original source... Go to PubMed...

    Return to the content