MMSL 2012, 81(2):68-75 | DOI: 10.31482/mmsl.2012.009

EFFECT OF SOMAN ON JNK AND P38 MITOGEN ACTIVATED PROTEIN KINASE (MAPK) PATHWAYSOriginal article

Jaroslav Pejchal ORCID...1*, Jan Österreicher2, Jiří Kassa ORCID...3, Václav Mařák1, Aleš Tichý ORCID...2, Zuzana Šinkorová ORCID...2, Lenka Zárybnická ORCID...2, Klára Kubelková ORCID...1, Kamil Kuča ORCID...1
1 Center of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic
2 Department of Radiation Biology, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic
3 Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, 500 01 Hradec Kralove, Czech Republic

The purpose of our study was to examine an early activation of JNK and p38 mitogen activated protein kinases (MAPK) and their substrate c-Myc after soman poisoning in order to enlighten the pathogenetic mechanism of nerve agent-induced non-specific effects. Male Wistar rats were intramuscularly poisoned by soman (60 μg.kg-1 - 70% LD50). Samples were taken 4, 24, and 72 hours after poisoning, immunohistochemically stained and phospho-JNKThr-183/Tyr-185, phospho-p38Thr180/Tyr182, and phospho-c-MycThr58/Ser62 expressions were measured using a computer Image analysis in apical and cryptal enterocytes of the colon transversum. We observed decreased phospho-JNK in apical enterocytes 4 and 24 h after poisoning and increased phospho-JNK in cryptal and apical enterocytes 72 h after intoxication. Phospho-p38 dropped significantly in the apical compartment 72 h after soman poisoning. An activation of c-Myc decreased in both apical and cryptal compartment 4 and 24 h after soman intoxication, while increased in both compartments 72 h after poisoning. Soman poisoning seems to temporarily suppress promitotic pathways of proliferating cryptal cells and causes delayed activation of JNK stress signaling pathway.

Keywords: soman; JNK, p38; c-Myc; enterocyte; rat; image analysis

Received: February 19, 2012; Revised: May 18, 2012; Published: June 8, 2012  Show citation

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Pejchal, J., Österreicher, J., Kassa, J., Mařák, V., Tichý, A., Šinkorová, Z., ... Kuča, K. (2012). EFFECT OF SOMAN ON JNK AND P38 MITOGEN ACTIVATED PROTEIN KINASE (MAPK) PATHWAYS. MMSL, 81(2), 68-75. doi: 10.31482/mmsl.2012.009
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