MMSL, 2012 (vol. 81), issue 2
FRANCISELLA TULARENSIS - 100 YEARS: TULAREMIA RESEARCH IN FORMER CZECHOSLOVAKIA AND IN THE CZECH REPUBLICReview article
Aleš Macela, Jiří Stulík, Zuzana Kročová, Michal Kroča, Klára Kubelková
MMSL 2012, 81(2):46-55 | DOI: 10.31482/mmsl.2012.006 
The history of national tularemia research started in 1936 when the first outbreak was recognized in south-east Moravia. Since then in average about one hundred cases have been recorded annually. As tularemia was endemic in former Czechoslovakia, three research groups which concentrated on this disease were formed during decades. The first two groups have worked from sixties and were associated with Jiri Libich (Prague) and Darina Gurycova (Bratislava). The third group which concentrated on the research of natural foci started during late seventies in Valtice (Zdenek Hubalek). The experimental research was, and still is, mainly associated with military...
CHARACTERIZATION OF SEABUCKTHORN OIL EMULSIONOriginal article
Snezana Cupara, Slobodan Jankovic, Ivana Arsic, Vanja Tadic, Vesna Jacevic
MMSL 2012, 81(2):56-60 | DOI: 10.31482/mmsl.2012.007
External application of seabuckthorn oil (Hippophae rhamnoides L.) is difficult due to its liquid state in spite of its benefits for damaged skin. In order to overcome this inadequacy the semisolid emulsion with seabuckthorn oil was prepared. Previous research showed that this emulsion possessing an enhanced structure with liquid crystals showed a higher wound healing potential than seabuckthorn oil. The aim of this investigation was to characterize suitability of this emulsion for topical use. The emulsion was prepared by combining emulsifiers that form liquid crystals. Two different quantities of seabuckthorn oil were incorporated. Samples...
GYROMITRIN, MUSHROOM TOXIN OF GYROMITRA SPP.Review article
Jiří Patočka, René Pita, Kamil Kuča
MMSL 2012, 81(2):61-67 | DOI: 10.31482/mmsl.2012.008
Gyromitra esculenta (Persoon ex Fries) mushrooms have been responsible for severe intoxications and even deaths. Clinical data are characterized primarily by vomiting and diarrhea and after a while by jaundice, convulsions and coma. Other Gyromitra species which may be of concern are G. fastigiata and G. gigas; nevertheless, recent advances in chromatography, biochemistry and toxicology have established that other species within the Ascomycetes may also prove toxic. Their toxins, mainly gyromitrin (N-methyl-N-formyl-N-acetyl-hydrazone) and their higher homologues are converted in the milieu of human stomach into N-methyl-N-formylhydrazine...
EFFECT OF SOMAN ON JNK AND P38 MITOGEN ACTIVATED PROTEIN KINASE (MAPK) PATHWAYSOriginal article
Jaroslav Pejchal, Jan Österreicher, Jiří Kassa, Václav Mařák, Aleš Tichý, Zuzana Šinkorová, Lenka Zárybnická, Klára Kubelková, Kamil Kuča
MMSL 2012, 81(2):68-75 | DOI: 10.31482/mmsl.2012.009
The purpose of our study was to examine an early activation of JNK and p38 mitogen activated protein kinases (MAPK) and their substrate c-Myc after soman poisoning in order to enlighten the pathogenetic mechanism of nerve agent-induced non-specific effects. Male Wistar rats were intramuscularly poisoned by soman (60 μg.kg-1 - 70% LD50). Samples were taken 4, 24, and 72 hours after poisoning, immunohistochemically stained and phospho-JNKThr-183/Tyr-185, phospho-p38Thr180/Tyr182, and phospho-c-MycThr58/Ser62 expressions were measured using a computer Image analysis in apical and cryptal enterocytes...
SYNTHESIS OF THE ISOQUINOLINIUM SALTS DIFFERING IN THE LENGTH OF THE SIDE ALKYLATING CHAINOriginal article
Jan Marek, Petr Stodůlka, Ondřej Soukup, Kamil Musílek, Jiří Cabal, Kamil Kuča
MMSL 2012, 81(2):76-81 | DOI: 10.31482/mmsl.2012.010
Cationic tensides are a widespread group of surface active agents. These compounds have lots of applications in various branches of industry and research. Quaternary isoquinolinium salts differing in alkyl chain length are members of a cationic surfactant group with quaternary nitrogen in its structure. The members of this group can be used as micellar catalysts or disinfectants. Decontamination (chemical warfare agents) or disinfection (bacteria or fungi) for very similar compounds was described several times. In this work, the preparation of isoquinoline-derived cationic surfactants differing in the length of the side alkylating chain from C8 to...
NATURAL PHOSPHOR ESTER CHOLINESTERASE INHIBITORSLetter to the editor
Georg Petroianu
MMSL 2012, 81(2):82-83 | DOI: 10.31482/mmsl.2012.011
It was with great interest and pleasure that I read the contribution of Patocka et al. in the MMSL reviewing the literature on anatoxin-A(s) an Organophosphorus Cholinesterase Inhibitor (OP) of natural origin produced by cyanobacteria (formerly identified as algae). Anatoxin-A(s), a phosphate ester of imidazole (Figure 1), is a very interesting substance that lacking the ability to cross the bloodbrain-barrier selectively and irreversibly inhibits the cholinesterases ...
A HISTORICAL OUTLINE OF THE TRAINING SYSTEM FOR FRENCH MILITARY PHYSICIANSLetter to the editor
Jean-Luc Perret
MMSL 2012, 81(2):84-86 | DOI: 10.31482/mmsl.2012.012
The story behind today's training-system for doctors serving in the French Armed Forces is embedded in a series of establishments each of which has left its mark in history, maintained the closest links with centres of improvisation, and instilled in its students a deep sense of attachment.It was at the very beginning of the 18th century that the first such establishment dedicated to military doctors appeared in France. It strove unceasingly to adapt itself not only to the needs of the military, but to the advancement and established use of knowledge pertinent to the changing conditions of military service.Since then, piecemeal evolution...
Job positionAnnouncement
Kamil Kuča
MMSL 2012, 81(2):87-88
Postdoctoral studies - development of new methods and evaluation of compound pharmacokinetics parameters & Postdoctoral studies -Position focused on synthetic work