MMSL 2012, 81(2):56-60 | DOI: 10.31482/mmsl.2012.007

CHARACTERIZATION OF SEABUCKTHORN OIL EMULSIONOriginal article

Snezana Cupara1*, Slobodan Jankovic1, Ivana Arsic2, Vanja Tadic2, Vesna Jacevic3
1 Medical Faculty, University of Kragujevac, Kragujevac, Republic of Serbia
2 Institute for Medicinal Plant Research "Dr. Josif Pančić", Belgrade, Republic of Serbia
3 National Poison Control Centre, Military Medical Academy, Belgrade, Republic of Serbia

External application of seabuckthorn oil (Hippophae rhamnoides L.) is difficult due to its liquid state in spite of its benefits for damaged skin. In order to overcome this inadequacy the semisolid emulsion with seabuckthorn oil was prepared. Previous research showed that this emulsion possessing an enhanced structure with liquid crystals showed a higher wound healing potential than seabuckthorn oil. The aim of this investigation was to characterize suitability of this emulsion for topical use. The emulsion was prepared by combining emulsifiers that form liquid crystals. Two different quantities of seabuckthorn oil were incorporated. Samples were prepared with 10% and 40% of seabuckthorn oil. Organoleptic characteristics were estimated visually and by smearing samples on a thin glass plate. Type of emulsion was determined by a conductometric method, while a pH value of the emulsion was measured by a pH meter. Samples of seabuckthorn emulsion were orange, semisolid, shiny, easily spreadable on skin, and the smear on the glass plate was homogeneous. There was an absence of smell and the emulsion could be rinsed by water after the application on skin, which is a desired characteristic of oil/water emulsions. Results of an electrical conductivity confirmed that an outer phase is water. Samples possesed an acceptable pH value for an external topical use. This research confirmed that constituents and a method used were suitable for preparing semisolid emulsion with seabuckthorn oil. Organoleptic properties, a pH value and a type of obtained emuslion appear to be adequate for topical use.

Keywords: Seabuckthorn oil; Characteristization; Emulsion; Topical use

Received: November 11, 2011; Revised: May 15, 2012; Published: June 8, 2012  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Cupara, S., Jankovic, S., Arsic, I., Tadic, V., & Jacevic, V. (2012). CHARACTERIZATION OF SEABUCKTHORN OIL EMULSION. MMSL81(2), 56-60. doi: 10.31482/mmsl.2012.007
Download citation

References

  1. Rousi, A. The genus Hippophae L. A taxonomic study. Annales. Botanici. Fennici. 1971, 8, 177-227.
  2. Rati, V.I.; Rati, L. Catina alba in explotatii agricole. Ministerul. Agriculturii. padurilor apelor si mediului, Bucuresti 2003.
  3. Tucakov, J. Herbal teraphy - phytotherapy. Rad. Belgrade 1990, p 532. (in Serbian)
  4. Sarić, M. Medical plants in Republic of Serbia. Serbian Academy of science and art, Department of natural and mathematic science. Book. 65. Belgrade 1989, p 307-309. (in Serbian)
  5. Ganju, L.; Padwad, Y.; Singh, R.; Karan, D.; Chanda, S.; Chopra, M.K.; Bhatnagar, P.; Kashyap, R.; Sawhney, R.C. Anti-inflammatory activity of seabuckthorn (Hippophae rhamnoides L.) leaves. Int. Immunopharmacol. 2005, 5, 1675-1684. Go to original source... Go to PubMed...
  6. Saggu, S.; Divekar, H.M.; Gupta, V.; Sawhney, R.C.; Banerjee, P.K.; Kumar, R. Adaptogenic and safety evaluation of seabuckthorn (Hippophae rhamnoides) leaf extract: A dose dependent study. Food. Chemic. Toxicol. 2007, 45, 609-617. Go to original source... Go to PubMed...
  7. Upadhyay, N.K.; Kumar, M.S.; Gupta, A. Antioxidant, cytoprotective and antibacterial effects of Sea buckthorn (Hippophae rhamnoides) leaves. Food. Chem. Toxicol. 2010, 48, 3443-3448. Go to original source... Go to PubMed...
  8. Mironov, V.A.; Guseva-Donskaya, T.N.; Dubrovina, Yu.Yu.; Osipov, G.A.; Shabanova, E.A.; Nikulin, A.A.; Amirov, N.Sh.; Trubitsina, I.G. Chemical composition and biological activity of extracts from sea buckthorn fruit components. Khimiko. - Farmatsevticheskii. Zhurnal. 1989, 23, 1357-1364.
  9. Xu, M.Y.; Sun, X.X.; Tong, W.X. Medical research and development of sea buckthorn. Hippophae. 1994, 7, 32-40.
  10. Li, T.S.C.; Schroeder, W.R. Sea Buckthorn (Hippophae rhamnoides L.): A multipurpose plant. Hort. Tech. 1996, 6, 370-380. Go to original source...
  11. Yang, B.; Kallio, H.; Effects of Harvesting Time on Triacylglycerols and Gycerophospholipids of Sea Buckthorn (Hippophae rhamnoides L.) Berries of Different Origins. J. Food. Comp. Anal. 2002, 15, 143-157. Go to original source...
  12. Yang, B.; Kallio, H.Composition and physiological effects of sea buckthorn (Hippophae) lipids. Tren. Food. Sci. Techn. 2002, 13, 160-167. Go to original source...
  13. Yang, B.; Kalimo, K.; Tahvonen, R.; Mattila, L.; Katajisto, J.; Kallio, H. Effects of dietary supplementation with sea buckthorn (Hippophae¨ rhamnoides) seed and pulp oils on the fatty acid composition of skin glycerophospholipids of patients with atopic dermatitis. J. Nutr. Biochem. 2000, 11, 338-340. Go to original source... Go to PubMed...
  14. Upadhyay, N.K.; Kumar, R.; Mandotra, S.K.; Meena, R.N.; Siddiqui, M.S.; Sawhney, M.S.; Gupta, A. Safety and healing efficacy of Sea buckthorn (Hippophae rhamnoides L.) seed oil on burn wounds in rats. Food. Chem. Toxicol. 2009, 47, 1146-1153. Go to original source... Go to PubMed...
  15. Majewska, I.; Gendaszewska-Darmach, E. Proangiogenic activity of plant extracts in accelerating wound healing - a new face of old phytomedicines. Acta Biochim. Pol. 2011, 58, 449-460. Go to original source... Go to PubMed...
  16. Suryakumar, G.; Gupta, A. Medicinal and therapeutic potential of Sea buckthorn (Hippophae rhamnoides L.). J. Ethnopharmacol. 2011, 138, 268-278. Go to original source... Go to PubMed...
  17. Cupara, M.S.; Ninkovic, B.M.; Knezevic, G.M.; Vuckovic, M.I.; Jankovic, M.S. Wound healing potential of seabuckthorn oil topical emulsion. Healthmed. 2011, 5, 1218-1223.
  18. Reminton: The Science and Practice of Pharmacy. Baltimor, Philadelphia: Lippincot &Willkins 2000, p 739-740.
  19. Stone, H.; Sidel, J. Sensory evaluation for skin products. Cosm. Toil. 1986, 101, 45-50.
  20. Wiechester, J.; Wortel, V. Making sense of sensory data. Cosm. Toil. 2000, 115, 37-45.
  21. Sehn, E.; Hernandes, L.; Franco, S.L.; Goncalves, C.C.M.; Baesso, M.L. Dynamics of reepithelisation and penetration rate of a bee propolis formulation during cutaneous wound healing. Anal. Chim. Acta. 2009, 635, 115-120. Go to original source... Go to PubMed...
  22. Otto, A.; Plessis, J.; Wiechers, J.W. Formulation effects of topical emulsion on transdermal and dermal delivery. Int. J. Cosmet. Sci. 2009, 32, 1-19. Go to original source... Go to PubMed...
  23. Al-Bawab, A.; Friberg, S.E. Some pertinent factors in skin care emulsion. Adva.Coll. Inter. Sci. 2006, 123-126, 313-322. Go to original source... Go to PubMed...
  24. Roberts, M.S.; Walker, M. Water: the most natural penetration enhancer. In: Pharmaceutical Skin Penetration Enhancement (Walters, K.A. and Hadgraft, J., eds.), Marcel Dekker, New York 1993, p 1-30.
  25. Muller-Goymann, CC. Liquid crystals in emulsions, creams and gels containing ethoxylated alcohols as surfactants. Pharm. Res. 1984, 4, 154-158. Go to original source... Go to PubMed...
  26. Suzuki, T.; Tsutsumi, H.; Ishida, A. Secondary droplet emulsion: mechanism and effects of liquid crystal formation in o/w emulsion. J. Disper. Sci. Techn. 1984, 5, 119-141. Go to original source...
  27. European Pharmacopoeia. 3 rd Edition. Council of Europe, Strasbourg 1997, p 1440.