MMSL 2021, 90(1):2-11 | DOI: 10.31482/mmsl.2021.001

THE EFFECT OF HYPERBARIC OXYGEN THERAPY ON ACUTE WOUND HEALING IN RABBITS: AN EXPERIMENTAL STUDY AND HISTOPATHOLOGICAL ANALYSISOriginal article

Jakub Tlapák ORCID...1,2*, Petr Chmátal ORCID...1, Jaroslav Pejchal ORCID...2, Boris Oniščenko ORCID...1,3, Věra Radochová ORCID...2, Petr Došel1, Jiří Páral ORCID...2, Petr Lochman ORCID...2
1 The Institute of Aviation Medicine, Prague, Czech Republic
2 Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic
3 Third Faculty of Medicine, Charles University in Prague, Prague, Czech Republic

Background: The goal of our research is to show the effects and impacts of hyperbaric oxygen therapy (HBOT) on acute model wounds in animal subjects.

Methods: Three experimental groups were created using injured rabbits (N=36)—randomly divided into three groups (N=12 per group). One group was treated only with standard wound care management. Two groups were additionally treated with HBOT either once or twice a day. The wounds were surgical, uninfected, and in healthy animal test subjects. We compared the immunohistochemical and histological parameters in 4-, 7- and 10-day intervals.

Results: The detection of epidermal leaf parameters, the number of microabscesses, the Histopathological Superficial Epithelium Healing Score, Connective Tissue Healing Score, Histopathological Acute Inflammation Score and Total Histopathological Wound Healing Score all showed significant changes between time intervals within the individual groups.

Conclusion: The results did not show that HBOT had a significant effect on the healing process of uncomplicated acute wounds.

Keywords: Hyperbaric oxygen; Wound healing; Animal models; Adjunctive treatment

Received: September 23, 2020; Revised: December 9, 2020; Accepted: January 4, 2021; Prepublished online: January 19, 2021; Published: March 5, 2021  Show citation

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Tlapák, J., Chmátal, P., Pejchal, J., Oniščenko, B., Radochová, V., Došel, P., Páral, J., & Lochman, P. (2021). THE EFFECT OF HYPERBARIC OXYGEN THERAPY ON ACUTE WOUND HEALING IN RABBITS: AN EXPERIMENTAL STUDY AND HISTOPATHOLOGICAL ANALYSIS. MMSL, 90(1), 2-11. doi: 10.31482/mmsl.2021.001
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    References

    1. Sureda A, Batle JM, Martorell M, Capó X, Tejada S, Tur JA, Poms A. Antioxidant Response of Chronic Wounds to Hyperbaric Oxygen Therapy. PLoS One. 2016;11(9):e0163371. Go to original source... Go to PubMed...
    2. Francis A, Baynosa RC. Hyperbaric Oxygen Therapy for the Compromised Graft or Flap. Adv Wound Care 2017;6:23-32. Go to original source... Go to PubMed...
    3. Sander AL, Henrich D, Muth CM, Marzi I, Barker JH, Frank JM. In vivo effect of hyperbaric oxygen on wound angiogenesis and epithelialization. Wound Repair Regen 2009;17(2):179-84. Go to original source... Go to PubMed...
    4. Hatibie MJ, Islam AA, Hatta M, Moenadjat Y, Susilo RH, Rendy L. Hyperbaric Oxygen Therapy for Second-Degree Burn Healing: An Experimental Study in Rabbits. Adv Skin Wound Care. 2019;32(3):1-4. Go to original source... Go to PubMed...
    5. Hadanny A, Efrati S. The Hyperoxic-Hypoxic Paradox. Biomolecules. 2020;10(6):958. Go to original source... Go to PubMed...
    6. Memar MY, Yekani M, Alizadeh N, Baghi HB. Hyperbaric oxygen therapy: Antimicrobial mechanisms and clinical application for infections. Biomed Pharmacother. 2019;109:440-447. Go to original source... Go to PubMed...
    7. De Smet GHJ, Kroese LF, Menon AG, Jeekel J, van Pelt AWJ, Kleinrensink GJ, Lange JF. Oxygen therapies and their effects on wound healing. A systematic review. Wound Repair Regen. 2017;25:591-608. Go to original source... Go to PubMed...
    8. Santema TJ, Stoekenbroek RM, van Steekelenburg KC, van Hulst RA, Koelemay MJ, Ubbink DT. Economic outcomes in clinical studies assessing hyperbaric oxygen in the treatment of acute and chronic wounds. Diving Hyperb Med. 2015;45(4):228-34. Go to PubMed...
    9. Roje Z, Roje Ž, Eterović D, Družijanić N, Petričević, Roje T, Čapkun V. Influence of Adjuvant Hyperbaric Oxygen Therapy on Short-term Complications During Surgical Reconstruction of Upper and Lower Extremity War Injuries: Retrospective Cohort Study. Croat Med J. 2008;49:224-32. Go to original source... Go to PubMed...
    10. Tlapák J, Chmátal P, Oniščenko B, Pavlík V, Došel P, Páral J, Lochman P. The effect of hyperbaric oxygen therapy on gene expression: microarray analysis on wound healing. Undersea Hyperb Med. 2020;47(1):31-37. Go to original source... Go to PubMed...
    11. Sultana J, Molla MR, Kamal M, Shahidullah M, Begum F, Bashar MA. Histological differences in wound healing in maxillofacial region in patients with or without risk factors. Bangladesh J Pathol 2009;24:38.
    12. Parnell LKS, Volk SW. The Evolution of Animal Models in Wound Healing Research: 1993-2017. Adv Wound Care. 2019;8(12):692-702. Go to original source... Go to PubMed...
    13. Holy R, Prazenica P, Stolarikova E, Dosel P, Fundova P, Kovar D, Astl J. Hyperbaric oxygen therapy in tinnitus with normal hearing in association with combined treatment. Undersea Hyperb Med. 2016;43(3):201-5.
    14. Hadanny A, Abbott S, Suzin G, Bechor Y, Efrati S. Effect of hyperbaric oxygen therapy on chronic neurocognitive deficits of post-traumatic brain injury patients: retrospective analysis. BMJ Open 2018;8(9):e023387. Go to original source... Go to PubMed...
    15. Jain KK. HBO Therapy in Infections. In: Jain, KK, ed. by Textbook of Hyperbaric Medicine. 5th ed. Toronto: Hogrefe and Huber Publishers; 2009.p.135-148.
    16. Hájek M. Akutní traumatická ischemie a drtivá poranění končetin. In: Hájek M., ed. by Hyperbarická medicína. Praha: Mladá fronta; 2017.p.301-313.
    17. Crowe DT. Hyperbaric oxygen therapy in veterinary medicine: a case series at Carson-Tahoe veterinary hospital. Hyperbaric Med Today. 2000;1(3):13-15.
    18. Mensack S, Murtaugh R. Oxygen toxicity. Compend Contin Educ Pract Vet. 1999;21(4):341-351.
    19. Millar I, McGinnes R, Williamson O, Lind F, Jansson KA, Hajek M, Smart D, Fernandes T, Miller R, Myles P, Cameron P. Hyperbaric oxygen in Lower Limb Trauma (HOLLT); protocol for a randomised controlled trial. BMJ Open. 2015;5(6):e008381. Go to original source... Go to PubMed...
    20. Weber R, Silver A, Williams SJ, Stephenson L, Usera PC, Zhang F, Tian H, Yang W, Wang WZ, Fang XH, Zamboni WA, Baynosa R. Random flap survival with hyperbaric oxygen: daily versus twice-daily treatments. Undersea Hyperb Med. 2018;45(2):157-164. Go to original source...
    21. Lemo N, Marignac G, Reyes-Gomez E, Lilin T, Crosaz O, Ehrenfest DMD. Cutaneous reepithelialization and wound contraction after skin biopsies in rabbits: a mathematical model for healing and remodelling index. Veterinarski Arhiv. 2010;80(5):637-652
    22. Valizadeh R, Hemmati AA, Houshmand G, Bayat S, Bahadoram M. Wound healing potential of Althaea officinalis flower mucilage in rabbit full thickness wounds. Asian Pac J Trop Biomed 2015;5(11):937-943 Go to original source...
    23. Latimer CR, Lux CN, Roberts S, Drum MG, Braswell C, Sula MJM. Effects of hyperbaric oxygen therapy on uncomplicated incisional and open wound healing in dogs. Veterinary Surgery. 2018;47:827-836. Go to original source... Go to PubMed...
    24. Mathieu D., Marroni A, Kot J. Tenth European Consensus Conference on Hyperbaric Medicine: recommendations for accepted and non-accepted clinical indications and practice of hyperbaric oxygen treatment. Diving Hyperb Med. 2017;47:24-32. Go to original source... Go to PubMed...

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