MMSL, 2011 (vol. 80), issue 4
COMPARISON OF POTENTIAL CYTOTOXICITY AND GENOTOXICITY OF SELECTED ANTIDOTES AGAINST ORGANOPHOSPHATES INHIBITING ACETYLCHOLINESTERASEOriginal article
Hana Svobodová, Petr Jošt, Rudolf Štětina
MMSL 2011, 80(4):142-149 | DOI: 10.31482/mmsl.2011.020 
Organophosphorous compounds cause fatal intoxication based on inhibition of acetylcholinesterase, an essential enzyme of neurosynapses and neuromuscular junctions. There is an obvious need to develop appropriate treatment against them due to their application in agriculture and chemical industry or their misuse in terrorist or war attack.In the Czech army some medicaments have been established to be used against this poisoning; Obidoxime, Methoxime, Atropine and Benactyzine. In present in vitro study we focused on potential cytotoxic and genotoxic effect evaluation of these drugs by the clonogenic and comet assay, respectively.Obtained results...
MULTIPLE MECHANICAL AND THERMAL BLAST INJURY IN CIVILIAN INDUSTRIAL SETTING - POSSIBLE PARALLEL TO THE BATTLEFIELD BLAST SYNDROME TYPE INJURIESCase report
Leo Klein, Eduard Havel, Karel Šmejkal, Jaroslav Cerman, Frantisek Hošek, Miloslav Hronek
MMSL 2011, 80(4):150-158 | DOI: 10.31482/mmsl.2011.021
A 43-year-old man, injured during a factory explosion on January 4, 2010, was transported to the nearest hospital. Volume resuscitation started, subclavian vein cannulation, chest tube, and covering of burns were performed. The intubated and ventilated patient was transferred by helicopter to our hospital. The leading diagnoses were: haemorrhagic - traumatic and burns shock, haemoperitoneum, pneumothorax, subtotal amputation of the left distal crus/feet, burns over 40 % of the body surface, right calcaneus fracture etc. Preliminary circulatory stabilisation with discontinuation of norepinephrine infusion was achieved within 10 hours, by excessive positive...
INTERLABORATORY COMPARATIVE TESTS OF BIOLOGICAL LABORATORIES OF THE NATO ARMIESOriginal article
Libor Píša, Radoslav Krupka, Veronika Formánková, Věra Neubauerová, Jiří Dresler, Martin Hubálek
MMSL 2011, 80(4):159-168 | DOI: 10.31482/mmsl.2011.022
One of the key requirements of the biodefense system of the Czech Armed Forces is a capability to identify the biological warfare agents (BWA). In this regard the Central Military Health Institute that is responsible for the biodefense in the Czech Armed Forces took part in 10th Annual international comparative exercise of the NATO (North Atlantic Treaty Organization) military biological laboratories. The aim of this test was the identification of Bacillus anthracis in unknown samples which were contaminated by different disinfectants. Real-time polymerase chain reaction (PCR) was chosen as optimal method for this exercise because...
ROLE OF γ-H2AX IN DNA-DAMAGE RESPONSE AND ITS POSSIBLE CLINICAL APPLICATIONSReview article
Eva Novotná, Aleš Tichý, Karolína Foltánová, Jiřina Vávrová
MMSL 2011, 80(4):169-177 | DOI: 10.31482/mmsl.2011.023
The integrity of the human genome is constantly threatened by exogenous or endogenous genotoxic agents that cause DNA damage. The ionizing radiation (IR)-induced DNA double-strand breaks (DSBs) are considered as the most deleterious forms of DNA damage which could lead to genomic instability and to cancer development, if left unrepaired. The DNA damage response (DDR) is comprised of a network of proteins that cooperate to regulate cell cycle progression and repair of DNA lesions. Our understanding of molecular basis of repair processes and of functions of repair proteins, as well as understanding of chromatin modifications may provide new possibilities...
OXIMES AS INHIBITORS OF ACETYLHOLINESTERASE - A STRUCTURE-ACTIVITY RELATIONSHIP (SAR) STUDYOriginal article
Vendula Šepsová, Jana Žďárová Karasová, Filip Zemek, Brian J. Bennion, Kamil Kuča
MMSL 2011, 80(4):178-186 | DOI: 10.31482/mmsl.2011.024
Acetylcholinesterase (AChE) reactivators (oximes) are generally used as antidotes in case of nerve agent poisoning. Because of their affinity to AChE, they may also act as weak inhibitors of AChE. Their inhibition potency against AChE was determined by an in vitro method based on the interaction between AChE and oxime reactivator in the concentration range 10-1 to 10-8 M. We used eel AChE for these assays. We found that AChE inhibition strongly depends on the oxime structure. The aim of the present study is to describe the structure-activity relationship (SAR) between oxime structure and inhibition of AChE. AChE reactivators...
THE 11th CONFERENCE OF THE CZECH SOCIETY OF MILITARY PHYSICIANS, PHARMACISTS AND VETERINARIANSLetter to the editor
Leo Klein
MMSL 2011, 80(4):187-188
The 11th Conference of the Society of Military Physicians, Pharmacists and Veterinarians took place at the Faculty of Military Health Sciences, University of Defence, in Hradec Králové, on the 18th and 19th October, 2011. The Society is a member of the Czech J. E. Purkinje Medical Association.The main topics of the Conference were a) progress in diagnostics and treatment-procedures by military physicians engaged in primary care or practising in medical establishments b) practical problems of organization and command- and - control within the Service c) scientific activities and research in military health-care d)...
Job positionAnnouncement
Kamil Kuča
MMSL 2011, 80(4):189-190
Post-doctoral position in the research area of "Drug Development" & Post-doctoral position in the research area of "Clinical Proteomics"