MMSL, 2013 (vol. 82), issue 3
MODULAR CONCEPTION OF MASS PERSONNEL DECONTAMINATIONOriginal article
Petr Žuja, Zdeněk Skaličan, Pavel Otřísal
MMSL 2013, 82(3):94-101 | DOI: 10.31482/mmsl.2013.014 
Personnel decontamination belongs to one of the crucial measurements performed to personnel's protection within hazard management after either employment of Weapons of Mass Destruction or leakage of Toxic Industrial Materials. Suitable personnel, technical and material conditions corresponding to current demands and trends are necessary to create its realization in this area. Knowledge gained within solution problems of personnel decontamination, casualty decontamination, immobile people decontamination, decontamination of personnel weapons, selected Individual Protective Equipment, personnel garments, accoutrements and other materials with the usage...
MODELING THE BINDING OF CWAs TO AChE AND BuChEOriginal article
Brian J. Bennion, Edmond Y. Lau, Jean-Luc Fattebert, Patrick Huang, Eric Schwegler, William Corning, Felice C. Lightstone
MMSL 2013, 82(3):102-114 | DOI: 10.31482/mmsl.2013.015
Traditional chemical weapon agents (CWAs) are known to bind acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE). Their lethality is known to be different for different mammalian species. We have modeled the binding affinity of CWAs to AChE and BuChE in human, rabbit, rat and mouse using molecular docking and free energy calculations. Through molecular docking we are able to correctly bind the CWAs at the active site. Using molecular mechanics generalized Born surface area (MMGBSA) calculations, we determined the binding free energy in the active site. Through these calculations, we observe that correct orientation at the active site is critical...
MILITARY INCAPACITATING AGENT BZ (3-QUINUCLIDINYL BENZILATE) - PAST, PRESENT AND FUTUREReview article
Jan Misík
MMSL 2013, 82(3):115-119 | DOI: 10.31482/mmsl.2013.016
The military incapacitating agent BZ (3-quinuclidinyl benzilate) is an anticholinergic compound that acts at both, the peripheral and central nervous system. Effects of the agent were discovered during the Cold War and BZ became one of the most potent anticholinergic psychomimetics, characterized by low effective doses causing long-term incapacitation. History, characteristics and potential use of BZ in behavioral research are discussed throughout this review.
SELECTIVE MONITORING OF ENZYMATIC ACTIVITY OF ACETYLCHOLINESTERASE BY FLOW INJECTION ANALYSIS WITH MASS SPECTROMETRIC DETECTIONOriginal article
Vlastimil Dohnal, Lucie Dohnalová, Daniel Jun, Kamil Kuča
MMSL 2013, 82(3):120-125 | DOI: 10.31482/mmsl.2013.017
Flow injection analysis (FIA) is an analytical method where the reaction mixture is injected into flow of liquid. The reaction product is monitored by a suitable detector such as ultraviolet/visible (UV/VIS) spectrophotometric or electrochemical detector. Mass spectrometric detectors (MS) are coming to be a standard equipment of analytical laboratories in the present time. This work is focused on application of FIA-MS instrumentation for monitoring of Ellman's reaction where both reactants (acetylthiocholine and 5,5'-dithiobis-2-nitrobenzoic acid, DTNB) and the reaction product (5-mercapto-2-nitrobenzoic acid) are monitored. This reaction is usually...
PROTECTIVE EFFECT OF AQUEOUS EXTRACTS OF AFRAMOMUM MELEGUETA ON γ-RADIATION-INDUCED LIVER DAMAGE IN MALE WISTAR RATSOriginal article
Nwozo S. O., Yakubu O. F., Oyinloye B. E.
MMSL 2013, 82(3):126-132 | DOI: 10.31482/mmsl.2013.018
This study was carried out to evaluate the radioprotective potential of aqueous extract of seeds of Aframomum melegueta (A.M.) against gamma radiation-induced (6Gy) liver damage in male Wistar rats. Thirty male rats were randomly distributed into six groups of five animals each and aqueous extract of A.M. was administered at a dose of 200 or 400 mg/kg b. wt., orally for 2 weeks prior to irradiation and 4 weeks after irradiation, when they were sacrificed. The hepatic antioxidant status; reduced glutathione (GSH), catalase (CAT) and glutathione peroxidase (GPx) as well as the extent of lipid peroxidation (LPO) were estimated. The activities of...
GLANCE BACK AT THE NATIONAL CONFERENCE "NUTRITION AND HEALTH 2012"Letter to the editor
Vladimír Pavlík, Eva Drahokoupilová
MMSL 2013, 82(3):133-134
Traditionally, the 16th national conference in a row on "Nutrition and Health" with international participation was held in the spa house Beethoven in Teplice on 18 - 19 September, 2012. The Teplice conference is the largest home conference focused on nutrition hygiene and primary prevention referred to a food intake. This year 40 specialists from the Czech Republic and Slovakia participated there. Specialities were represented mainly by physicians, nutritional therapists and regional hygiene stations workers. The Czech Medical Association, Third Faculty of Medicine, Charles University in Prague and the Health Institute located in Ústí nad Labem were...
REPORT ON AN INTERNSHIP AT THE BURNS CENTRE IN PITTSBURGH, USALetter to the editor
Igor Slaninka
MMSL 2013, 82(3):135-137
In the period between the 18th February and the 17th March, 2013, I had the opportunity of accepting an internship in The West Penn Hospital Burn Center, in Pittsburgh, Pennsylvania. During the internship, I shared in the usual medical activities including working in the ICU, standard wards, operating theatres, out-patient clinics as well as attending seminars in the Burns Centre or in the Plastic Surgery Department.The city of Pittsburgh is located in the State of Pennsylvania, in the North-East of the USA. The inner city has about 350,000 inhabitants, but the entire metropolitan area ...
IX. HRADEC VACCINATION DAYS, PARALLEL SYMPOSIUM FOR GENERAL PRACTITIONERSAnnouncement
Roman Chlíbek
MMSL 2013, 82(3):138
Congress centrum Aldis, Hradec Kralove, Czech RepublicOrganized by The Czech Vaccination Society, Faculty of Military Health Science University of Defence
CONFERENCE TOXCON 2013Letter to the editor
Martin Kuneš, Kamil Kuča
MMSL 2013, 82(3):139-140
18th Interdisciplinary Czech-Slovak Toxicology Conference