MMSL, 2011 (vol. 80), issue 2
METHOD OF STATIC DIFFUSION CELLS FOR ASSESSMENT OF PESTICIDES SKIN PERMEATIONOriginal article
Jan Misík, Růžena Pavlíková, Jiří Cabal, Ladislav Novotný, Kamil Kuča
MMSL 2011, 80(2):46-51 | DOI: 10.31482/mmsl.2011.007 
Usage and misusage of pesticides represent a health risk to military and civilian, especially to agricultural workers; also a possible terrorist threat is considered. The major route of low-volatility pesticides intoxication is percutaneous. Hence, skin permeation characteristics of pesticides are intensively investigated. In this study, in vitro measurement of skin permeation is presented on the example of pesticide paraoxon. Permeation experiments were performed in Franz-type of static diffusion cells using a pig skin. Paraoxon which permeated through the skin was determined enzymatically by modified Ellman's method. During 8 hours experiment,...
MICROBIAL AIR LOAD AT THE TRANSPLANT INTENSIVE CARE UNITOriginal article
Marie Vacková, Irena Hanovcová, Jan Smetana, Roman Chlíbek, Vanda Boštíková, Miroslav Špliňo
MMSL 2011, 80(2):52-57 | DOI: 10.31482/mmsl.2011.008
The air does contains microbial agents originally coming from the soil, water, plants or animals, including men. Temperature, light and humidity are the basic factors which has influence of microbial survival and abundance. Different microorganisms travel by aerial transmission and are involved in serious processes causing pneumonia and other diseases.In our study we decided to investigate microbial load in air at the Transplant Intensive Care Unit of the University Hospital of Hradec Králové, Czech republic for two years period. Air samples were taken from the patient's breathing zone in the single rooms. Air was sampled with Biotest RCS Plus air...
METHOD OPTIMIZATION FOR ACETYLCHOLINESTERASE MODULATORS-ALBUMIN INTERACTIONSOriginal article
Filip Zemek, Jana Žďárová Karasová, Kamil Musílek, Kamil Kuča
MMSL 2011, 80(2):58-64 | DOI: 10.31482/mmsl.2011.009
Plasma proteins such as human serum albumin (HSA) and alpha 1-acid glycoprotein (AGP) play an important role in the pharmacokinetic and pharmacodynamic properties of all endogenous or exogenous substances presented in the blood vessels. Their abundance in human blood not only helps to maintain hemostasis, but also makes them ideal candidates as transporters or possible depot reservoirs. Numerous methods e.g. equilibrium dialysis, ultrafiltration. ultracentrifugation, high-performance affinity chromatography were introduced to determine potential interactions with plasma proteins and possible quantification.In this work method was developed for the...
THE ROLE OF SONOGRAPHY IN EMERGENCY MEDICINEReview article
Jan Bydžovský
MMSL 2011, 80(2):65-71 | DOI: 10.31482/mmsl.2011.010
Sonography, thanks to the miniaturization, is increasingly widespread as a bedside examination technique in both hospital and prehospital emergency care. A number of protocols were set up for its effective and fast usage. The article briefly depicts this method and the requirements and ways of its application in the emergency care.
PELARGONIC ACID VANILYLLAMIDE (PAVA): RIOT CONTROL AGENTReview article
Jiří Patočka, Kamil Kuča
MMSL 2011, 80(2):72-79 | DOI: 10.31482/mmsl.2011.011
Riot control agents are highly potent sensory irritants of relatively low toxicity that produce dose and time-dependent acute site-specific toxicity. These compounds have been referred to as transient incapacitating agents or as lacrimators, and in common parlance they are known as "tear gases". These compounds interact pharmacologically with sensory nerve receptors associated with mucosal surfaces and the skin at the site of contamination, resulting in localized discomfort or pain with associated reflexes. This biological response, e.g. ocular irritation, results in pain in the eye and excess reflex lacrimation and blepharospasm. Riot control agents...
ON THE UNIVERSALITY OF OXIME HLö-7 - ANTIDOTE FOR CASE OF THE NERVE AGENT POISONINGOriginal article
Kamil Kuča, Kamil Musílek, Jana Karasová, Daniel Jun, Ondřej Soukup, Miroslav Pohanka, Kallol Kumar Ghosh, Martina Hrabinová
MMSL 2011, 80(2):80-84 | DOI: 10.31482/mmsl.2011.012
Searching for the universal oxime, which could be able to reactivate acetylcholinesterase inhibited by various nerve agents is still topic of high interest. In this contribution, oxime HLö-7, that was thoroughly discussed in the last decade, is evaluated . Its universality was tested in vitro using the rat brain homogenate as a source of the cholinesterases. The main members of the nerve agent family (tabun, sarin, soman, cyclosarin and VX) were used for this purpose. As shown, oxime HLö-7 was able to reactivate cholinesterases inhibited by all tested nerve agents with the exception of tabun. Hence, it could not be designated as the broad-spectrum...
THOMAS PROFT (ED.). MICROBIAL TOXINS: CURRENT RESEARCH AND FUTURE TRENDS. UNIVERSITY OF AUCKLAND PUBLISHING, NEW ZEALAND 2009, VIII + 192PP. ISBN: 978-1-904455-44-8.Book review
Jiří Patočka
MMSL 2011, 80(2):85-87
Toxins are important virulence factors responsible for microbial pathogenicity. Potential applications of toxin research extend beyond simply combating microbial virulence and include the development of novel anti-cancer drugs and other front-line medicines. No less important is the use of toxins as tools in cellular biology and neurobiology. Understanding toxin molecular and cellular biology is critical for the development of new anti-toxin strategies, particularly for those with bioterrorism capability. This well worked book not only provides a general overview of toxins but elucidates in detail recent molecular approaches, achievements and refreshing...
MILITARY DOCTORS WERE AWARDED HONORARY MEMBERSHIPLetter to the editor
Leo Klein
MMSL 2011, 80(2):88
On 18th May 2011, a ceremonial committee meeting of the Society of Military Physicians, Pharmacists and Veterinarians of the Czech Medical Association of J.E.Purkinje was held at the Faculty of Military Health Sciences of the University of Defence in Hradec Králové. On that occasion the merited and outstanding representatives of military medicine were awarded honorary membership diplomas. The founding members of the Society COL (ret.) Assoc. Prof. RNDr. et PhMr Vladimír Měrka, CSc., COL (ret.) Prof. MUDr. Miroslav Špliňo, DrSc, and COL (ret.) Prof. MUDr. Josef Fusek, DrSc, dr.h.c., were awarded. In this way, the Committee of the Society ...