MMSL 2011, 80(2):46-51 | DOI: 10.31482/mmsl.2011.007

METHOD OF STATIC DIFFUSION CELLS FOR ASSESSMENT OF PESTICIDES SKIN PERMEATIONOriginal article

Jan Misík ORCID...1*, Růžena Pavlíková ORCID...1, Jiří Cabal1, Ladislav Novotný2, Kamil Kuča ORCID...1,3
1 Department of Toxicology, Faculty of Military Health Sciences, University of Defence, Czech Republic
2 Faculty of Veterinary Medicine, University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic
3 Centre of Advanced Studies, Faculty of Military Health Sciences, University of Defence, Czech Republic

Usage and misusage of pesticides represent a health risk to military and civilian, especially to agricultural workers; also a possible terrorist threat is considered. The major route of low-volatility pesticides intoxication is percutaneous. Hence, skin permeation characteristics of pesticides are intensively investigated. In this study, in vitro measurement of skin permeation is presented on the example of pesticide paraoxon. Permeation experiments were performed in Franz-type of static diffusion cells using a pig skin. Paraoxon which permeated through the skin was determined enzymatically by modified Ellman's method. During 8 hours experiment, approx. 0.1 % of applied paraoxon has permeated through the skin. It was shown that pre-treatment by water simulating wet or sweated skin enhanced the paraoxon permeation.

Keywords: Pesticides; diffusion cell; skin permeation; paraoxon

Received: March 4, 2011; Revised: May 20, 2011; Published: June 10, 2011  Show citation

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Misík, J., Pavlíková, R., Cabal, J., Novotný, L., & Kuča, K. (2011). METHOD OF STATIC DIFFUSION CELLS FOR ASSESSMENT OF PESTICIDES SKIN PERMEATION. MMSL, 80(2), 46-51. doi: 10.31482/mmsl.2011.007
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