MMSL, 2013 (vol. 82), issue 4
MYCOPLASMA INFECTIONS IN HUMANSReview article
Radek Sleha, Vanda Boštíková, Miloslav Salavec, Pavel Boštík, Eva Slehová, Rudolf Kukla, Petra Mosio, Markéta Vydržalová, Jaroslava Mazurová
MMSL 2013, 82(4):142-148 | DOI: 10.31482/mmsl.2013.023 
Members of the genus Mycoplasma are parasitic bacteria that are widespread in nature. Several Mycoplasma species are important causative agents of various infections of mucosal surfaces in humans, especially in the urogenital or respiratory tracts. Pathogenetic mechanisms of mycoplasmas are intensively studied.The "gold" standard of mycoplasma detection is cultivation, which is very difficult and time-consuming. The other options for identifying mycoplasmas include direct antigen detection or molecular-biology methods, such as polymerase chain reaction, DNA-hybridization and sequencing.Mycoplasmas are naturally resistant to beta-lactam...
DETECTION OF 2-(DIALKYLAMINO)ETHANETHIOLS AND V-SERIES NERVE AGENTS IN WATER AND AQUEOUS SOLUTIONS USING FOLIN-CIOCALTEU REAGENTOriginal article
Vladimír Pitschmann, Lukáš Matějovský, Zbyněk Kobliha
MMSL 2013, 82(4):149-155 | DOI: 10.31482/mmsl.2013.024
A simple colorimetric tube detector for the detection of 2-(dialkylamino)ethanethiols and V-series nerve agents in water and in aqueous solutions is proposed. The detector is based on the reaction of the compounds with a chromogenic Folin-Ciocalteu reagent yielding a blue coloured reduction product. The detection limit of 2-(dialkylamino)ethanethiols is 2.5 mg/l, measurement range up to 250 mg/l. The detection limit of the V (VX) agent is 10 mg/l. The method allows V agents and their precursors and hydrolysis products to be easily distinguished from G-series nerve agents.
OPPOSITE TRENDS OF INCIDENCE OF GONORRHEA AND OTHER STIS IN THE EASTERN BOHEMIA DURING PAST 15 YEARSOriginal article
Miloslav Salavec, Vanda Boštíková, Petr Prášil, Jan Smetana, Miroslav Špliňo, Roman Chlíbek, Miroslav Louda, Pavel Boštík
MMSL 2013, 82(4):156-161 | DOI: 10.31482/mmsl.2013.025
The epidemiology of selected venereal diseases in the Czech Republic has been carefully evaluated for many years. This report containing data from the period 1981-2011 from the eastern Bohemia shows a sharp decrease in the incidence of gonorrhea in 1993-1994 and very low incidence thereafter with slightly higher prevalence in males. However, syphilis and genitourinary infection by Chlamydia trachomatis show entirely opposite trends. Also, for the similar number of diagnostic tests performed, chlamydia showed 10 fold higher ratio of positive cases. This underscores the changing epidemiology of STIs and necessity for adapting the reporting algorithms.
BIOLOGICALLY ACTIVE ALCOHOLS: CYCLIC ALCOHOLSReview article
Jiří Patočka, Kamil Kuča
MMSL 2013, 82(4):162-171 | DOI: 10.31482/mmsl.2013.026
The subjects of this article are cyclic alcohols with hydroxyl group bound directly to one carbon of the three- up to six-membered ring. They are thus predominantly secondary alcohols. These are substances frequently used as synthons in organic synthesis and many of them are important raw materials of chemical industry, such as cyclohexanol. Some cyclic alcohols were also found in nature, the bulk of them belong to the category of monoterpene substances. Many of them have biological activity, which is also discussed in this article.
THE POSSIBILITY OF IDENTIFYING SELECTED OPIOIDS BY SPECTRAL ANALYSISReview article
Romana Jelínková, Emil Halámek, Zbyněk Kobliha
MMSL 2013, 82(4):172-179 | DOI: 10.31482/mmsl.2013.027
The ability of the examined compounds to create ion pairs with the selected anionic dyes, allows their determination by the method of extraction spectrophotometry in the visible radiation. These ion pairs are extractable with an organic solvent immiscible with water. The procedure is based on protonisation of the studied compounds in acidic environment, and consequently their extraction in the form of associate with the anion of the acid dye into the organic phase, in this case in chloroform.Mentioned methods of analysis may be advantageously used for simple and express determination of fentanyl, alfentanil and remifentanil.
POTENTIAL ANTICLASTOGENIC EFFECT OF HYPERFORINReview article
Petronela Imreová, Eva Miadoková, Eliška Gálová, Stephka Chankova, Ivan Chalupa
MMSL 2013, 82(4):180-184 | DOI: 10.31482/mmsl.2013.028
Hyperforin is a prenylated phloroglucinol derivative which occurs in the plant Hypericum perforatum L. It has several medicinally important properties (antidepressant, anti-inflammatory, proapoptotic, antibacterial and antiangiogenic). To enable its medicinal use, it is necessary to investigate its potential genotoxic effect on human cell lines. We also observed an anticlastogenic effect of hyperforin towards the indirect mutagen benzo(a)pyrene. Benzo(a)pyrene is a widespread polycyclic aromatic hydrocarbon. We performed experiments on human tumor cell line HepG2, using the in vitro mammalian chromosome aberration test. We investigated two cytogenic...
TRANSDERMAL PENETRATION OF THE ACETYLCHOLINESTERASE REACTIVATOR HI-6 IN A RAT MODELOriginal article
Alžběta Kračmarová, Lucie Bartošová, Jana Žďárová Karasová, Filip Zemek, Jiří Bajgar
MMSL 2013, 82(4):185-188 | DOI: 10.31482/mmsl.2013.029
The objective of the experiment was to verify that HI-6 dimethanesulphonate (HI-6 DMS) is able to penetrate the skin in amounts sufficient to protect against organophosphate poisoning using a rat model. HI-6 2Cl is a major component of Transant, a transdermal patch, used as a protective agent against organophosphate intoxication in the Czech and Slovak armies, although there is little evidence that HI-6 would penetrate the skin in sufficient amounts. HI-6 DMS at a total amount of 127 mg or 635 mg was applied as a buffer solution on the Transant patch which was fixed on the back of the rat. Two, seven or twenty-four hours later, rats were sacrificed...
13th CONFERENCE ON MILITARY MEDICINEAnnouncement
Leo Klein
MMSL 2013, 82(4):189
Main topic: Section of Military Medical Services, Section of Treatment and Prevention, Section of Military Medical Research and Development, Poster Section
THE 10th CONFERENCE ON DISASTER MEDICINE - EXPERIENCE, PREPARATION, PRACTICEAnnouncement
Miroslav Procházka
MMSL 2013, 82(4):190
Main topics of the conference: Emergency and disaster planning and preparation, Disaster medicine - international experience, Major incidents and IRS drills - case studies, Current threats and risks, Ethnic, humanitarian and psycho-social aspects of major incidents, Pre-hospital and hospital emergency care - new trends, Preparation of health-care facilities for major incidents, Education, professional training and competence, Disaster medicine research, CBRNE, Catastrophe impact reduction, Civil and military aspects of disaster medicine.
MILITARY PHYSICIANS WILL REMEMBER PROFESSOR RADANA KÖNIGOVÁ, MD, CSc.Letter to the editor
Leo Klein
MMSL 2013, 82(4):191-192
On the 20th September, 2013, Czech medical community received a very sad announcement of the death of Professor Radana Königová, a prominent personality in the Czech medicine and whole society, respected authority within the international burns and medical societies. She passed away in the hospital where she had worked for more than 51 years, until the very last days, before orthopedic surgery she had had to undergo. Unfortunately, she did not return to her "second home", the Prague...