VZL 2005, 74(3):140-143

The Neural Stem Cells Radiosensitivity - The Valid Method for Consideration of Neural Stem Cells Radiosensitivity: Possible Tool for Estimation of External Influence on Neural Stem Cell

Jan Österreicher1, Jaroslav Mokrý2, Jana Karbanová2, Zdeňka Vilasová1
1 University of Defence, Department of Radiation Biology of Faculty of Military Health Sciences in Hradec Králové, Czech Republic
2 Charles University in Prague, Department of Histology and Embryology of Medical Faculty in Hradec Králové, Czech Republic

We studied radiosenzitivity of neural stem cells (NSCs) by widely used functional test and by method with adherent NSCs. NSCs were given 0-8 Gy, cultivated floating in medium (Group N) or finally adhered to polyornitine-coated Petri dishes (Group A). After 6 generation times neurospheres were fixed, stained and their numbers were counted. Finally adherent NSCs showed significantly higher radiosenzitivity than non-adherent NSCs from the dose of 0.5 Gy. Average D0 of group A was 1.85 Gy; average D0 of group N was counted as low as 0.96 Gy.We assume that the functional test widely used for influences determination to NSCs is not valid for NSCs radiosensitivity measurement. Additionally, NSCs radiosensitivity test should be performed with adherent NSCs.

Keywords: Neural stem cells; Radiosensitivity test; Adhesion

Received: March 23, 2005; Published: December 1, 2005  Show citation

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Österreicher, J., Mokrý, J., Karbanová, J., & Vilasová, Z. (2005). The Neural Stem Cells Radiosensitivity - The Valid Method for Consideration of Neural Stem Cells Radiosensitivity: Possible Tool for Estimation of External Influence on Neural Stem Cell. Vojenské Zdravotnické Listy, 74(3-4), 140-143
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