MMSL 2017, 86(1):11-16 | DOI: 10.31482/mmsl.2017.003

METHOD OPTIMIZATION FOR DETERMINATION OF DRUG SOLUBILITY LIMITOriginal article

Namrata Singh1,2*, Ondřej Soukup ORCID...2,3, Zdeněk Fišar4, Kamil Kuča ORCID...2,3
1 Department of Chemistry, Indian Institute of Technology Bombay, Mumbai-400076, India
2 Biomedical Research Centre, University Hospital Hradec Kralove, Sokolska 581, 500 05 Hradec Kralove, Czech Republic
3 Department of Toxicology, Faculty of Military Health Sciences, University of Defense, Trebesska 1575, Kralove, Czech Republic
4 Department of Psychiatry, Charles University, Kateřinská 32, 121 08 Prague 2, Czech Republic

In the early stage of drug development the solubility of drug candidate is the most crucial physicochemical parameter to be defined for the selection of lead compound. Conventional shake flask method of solubility determination has now been replaced with more precise measurements like ultraviolet absorption, nephelometry, nuclear magnetic resonance and potentiometry. The development of a simple, rapid, sensitive and precise spectrophotometric method for the routine quantitative determination of samples will definitely reduce unnecessary tedious sample preparations and the cost of materials and labour. This article accounts for the measurement of solubility limit of few selected drugs by spectrophotometry using dilution technique. This has been done to optimize the method for rapid and convenient determination of drug solubility limit. Concentration of saturated solution of drug was determined from the absorbance versus concentration plots of various diluted solutions of drug as per Beer-Lambert law and was reported as drug solubility limit.

Keywords: drugs; solubility limit; absorbance; spectrophotometry

Received: February 10, 2017; Revised: February 18, 2017; Published: March 10, 2017  Show citation

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Singh, N., Soukup, O., Fišar, Z., & Kuča, K. (2017). METHOD OPTIMIZATION FOR DETERMINATION OF DRUG SOLUBILITY LIMIT. MMSL, 86(1), 11-16. doi: 10.31482/mmsl.2017.003
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