MMSL 2022, 91(4):324-334 | DOI: 10.31482/mmsl.2022.012

CLOSTRIDIAL COLLAGENASE IMMOBILIZED ON CHITOSAN NANOFIBERS FOR BURN HEALINGOriginal article

Marcela Slovakova ORCID...1*, Renata Köhlerova ORCID...2, Petra Dvorakova1, Veronika Vanova1, Martina Spackova3, Marcela Munzarova ORCID...4
1 Faculty of Chemical Technology, University of Pardubice, Studentska 573, 532 19 Pardubice, Czech Republic
2 Department of Medical Biochemistry, Faculty of Medicine in Hradec Kralove, Charles University, Simkova 870, 500 03, Hradec Kralove, Czech Republic
3 Elmarco s.r.o., Svarovska 621, 460 01 Liberec, Czech Republic
4 Nano Medical s.r.o., Amperova 650, 463 12 Liberec, Czech Republic

This article describes the characterization and application of collagenase-based chitosan nanofiber membranes with rat burns. Electrospun chitosan nanofibers were functionalized with clostridial collagenase using carbodiimide chemistry. The immobilized collagenase was characterized by enzyme activity, kinetic constants, and dry storage stability measurements using a Pz-peptide substrate. The apparent kinetic constants KM and Vmax of immobilized collagenase showed a high affinity for the peptide substrate compared to the free enzyme. Drying of chitosan membranes with immobilized collagenase ensured 98 % stability of enzyme activity after rehydration. The effect of collagenase immobilized on chitosan nanofibers on the burn of the rat model was compared with a control treatment with chitosan nanofibers. The healing of the wound with both materials was terminated after 30 days at the same time, although the collagenase wound healed more rapidly during healing. The scar area size after the application of collagenase-containing chitosan nanofiber membranes was 31.6 % smaller than when only chitosan nanofibers were used.

Keywords: wound healing; immobilization; collagenase; chitosan; nanofibers

Received: October 13, 2021; Revised: January 6, 2022; Accepted: February 7, 2022; Prepublished online: March 17, 2022; Published: December 2, 2022  Show citation

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Slovakova, M., Köhlerova, R., Dvorakova, P., Vanova, V., Spackova, M., & Munzarova, M. (2022). CLOSTRIDIAL COLLAGENASE IMMOBILIZED ON CHITOSAN NANOFIBERS FOR BURN HEALING. MMSL, 91(4), 324-334. doi: 10.31482/mmsl.2022.012
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