MMSL 2023, 92(2):135-140 | DOI: 10.31482/mmsl.2022.032

EFFECTS OF FORMULATED TOPICAL NIFEDIPINE OINTMENT ON TGF–β AND ACCELERATION OF FACIAL SKIN WOUND HEALING IN RABBITSOriginal article

Wasan J. Al-Dabbagh ORCID...1*, Faehaa A. Al-Mashhadane ORCID...2, Ayad A. Al-Sarraj ORCID...2
1 Albatool Teaching Hospital, Alshifaa quarter Mosul, 41002, Ninevah Province, Iraq
2 Department of Dental Basic Sciences, University of Mosul, Alhadbaa Street, Mosul, 41002, Ninevah Province, Iraq

The purpose of this study was to evaluate the impact of 1% and 2% topical nifedipine ointments on TGF–β and the acceleration of facial skin wound healing in rabbits. Nifedipine ointments of 1% and 2% were prepared. Fifty healthy male rabbits were involved and distributed into two groups according to the study period: group A (7 days) and group B (14 days). Each group was subdivided into five groups (5 rabbits/group): Group I (Normal): rabbits did not undergo a surgical procedure and did not receive treatment; group II (negative control): rabbits had surgical wounds without treatment; group III (positive control): rabbits had surgical wounds with white petroleum treatment; group IV(nifedipine 1%): rabbits had surgical wounds with nifedipine 1% ointment treatment; and group V(nifedipine 2%): rabbits had surgical wounds with nifedipine 2% ointment treatment. Following euthanasia, blood samples (5 ml) were collected from all animals for TGF–β analysis using an ELISA kit. The TGF–β level in NFD 1% group was significantly higher on the 7th and14th day of the study associated with a higher rate of wound closure in comparison to other groups.

Conclusions: Nifedipine 1% ointment has beneficial value for improving wound healing, while nifedipine NFD 2% has no such effect.

Keywords: Nifedipine; Skin; Wound Healing; Transforming Growth Factor–β

Received: May 18, 2022; Revised: July 14, 2022; Accepted: July 15, 2022; Prepublished online: December 12, 2022; Published: June 2, 2023  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Al-Dabbagh, W.J., Al-Mashhadane, F.A., & Al-Sarraj, A.A. (2023). EFFECTS OF FORMULATED TOPICAL NIFEDIPINE OINTMENT ON TGF–β AND ACCELERATION OF FACIAL SKIN WOUND HEALING IN RABBITS. MMSL, 92(2), 135-140. doi: 10.31482/mmsl.2022.032
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Lauritano D, Moreo G, Vella FD, et al. Biology of Drug-Induced Gingival Hyperplasia: In Vitro Study of the Effect of Nifedipine on Human Fibroblasts. Applied Sciences. 2021;11(7):3287. https://doi.org/10.3390/app11073287 Go to original source...
    2. Mojiri H. The role of calcium channel blockers in wound healing. Iranian journal of basic medical sciences. 2018;21(12):1198. https://doi.org/10.22038/ijbms. 2018. 29753.7182 Go to original source... Go to PubMed...
    3. Tonsekar P, Tonsekar V. Calcium-Channel-Blocker-Influenced Gingival Enlargement: A Conundrum Demystified. Oral. 2021;1(3):236-249. https://doi.org/10.3390/oral1030023 Go to original source...
    4. Zolfagharnezhad H, Khalili H, Mohammadi M, et al. Topical nifedipine for the treatment of pressure ulcer: a randomized, placebo-controlled clinical trial. American Journal of Therapeutics. 2021;28(1):41-51. https://doi.org/10.1097/MJT.0000000000000936 Go to original source... Go to PubMed...
    5. Khan I, Rahman SU, Tang E, et al. Accelerated burn wound healing with photobiomodulation therapy involves activation of endogenous latent TGF-β1. Scientific reports. 2021;11(1):1-5. https://doi.org/10.1038/s41598-021-92650-w Go to original source... Go to PubMed...
    6. Du H, Jiang D, Song G, et al. Wound Healing Activity of Phage-Sisplayed TGF-β1 Model Peptide in Streptozotocin-Induced Diabetic Rats. International Journal of Peptide Research and Therapeutics. 2021;27(2):1079-1094. https://doi.org/10.1007/s10989-020-10152-1 Go to original source...
    7. Friciu M, Chefson A, Leclair G. Stability of Hydrocortisone, Nifedipine, and Nitroglycerine Compounded Preparations for the Treatment of Anorectal Conditions. The Canadian Journal of Hospital Pharmacy. 2016;69(4):329. https://doi.org/10.4212/cjhp.v69i4.1578 Go to original source... Go to PubMed...
    8. Katsinelos P, Papaziogas B, Koutelidakis I, et al. Topical 0.5% nifedipine vs. lateral internal sphincterotomy for the treatment of chronic anal fissure: long-term follow-up. International journal of colorectal disease. 2006;21(2):179-183. https://doi.org/10.1007/s00384-005-0766-x Go to original source... Go to PubMed...
    9. Asghar U, Khan R, Nigar Z, et al. Comparison of healing rate of anal fissure with 0.2% glyceryl trinitrate ointment versus 2% nifedipine ointment. PAFMJ. 2020;70(Suppl-1):91-94.
    10. Nazir T, Shakir L, Rahman ZU, et al. Hepatoprotective Activity of Foeniculum Vulgare Against Paracetamol Induced Hepatotoxicity in Rabbit. J. Appl. Pharm. 2020;12:2376-0354. Go to original source...
    11. Ricci F, Bresesti I, LaVerde PA, et al. Surfactant lung delivery with LISA and InSurE in adult rabbits with respiratory distress. Pediatric research. 2021;90(3):576-583. https://doi.org/10.1038/s41390-020-01324-2 Go to original source... Go to PubMed...
    12. Ragab GH, Zaki FM, Hassan FE, et al. Comparable Study of Different Materials (Silver Nanoparticles, PRP and its Mixture) That Enhance Surgical Excisional Skin Wound Healing in New Zealand Rabbits; Histopathological Evaluation. Annals of the Romanian Society for Cell Biology. 2021;25(6):15966-15975.
    13. Naji AH, Al-Watter WT, Taqa GA. The Effect of Xylitol on Bone Alkaline Phosphatase Serum Level and Bone Defect Diameter in Rabbits. Journal of Applied Veterinary Sciences. 2022;7(1):6-10. https://doi.org/10.21608/javs.2021.97815.1105 Go to original source...
    14. Brasileiro AC, Oliveira DC, Silva PB, et al. Impact of topical nifedipine on wound healing in animal model (pig). Jornal Vascular Brasileiro. 2020;19. https://doi.org/10.1590/1677-5449.190092 Go to original source... Go to PubMed...
    15. Hemmati AA, Forushani HM, Asgari HM. Wound healing potential of topical amlodipine in full thickness wound of rabbit. Jundishapur Journal of Natural Pharmaceutical Products. 2014;9(3). https://doi.org/10.17795/jjnpp-15638 Go to original source... Go to PubMed...
    16. Kiritsi D, Nyström A. The role of TGFβ in wound healing pathologies. Mechanisms of ageing and development. 2018;172:51-58. https://doi.org/10.1016/j.mad.2017.11.004 Go to original source... Go to PubMed...
    17. Li J, Chou H, Li L, et al. Wound healing activity of neferine in experimental diabetic rats through the inhibition of inflammatory cytokines and nrf-2 pathway. Artificial cells, nanomedicine, and biotechnology. 2020;48(1):96-106. https://doi.org/10.1080/21691401.2019.1699814 Go to original source... Go to PubMed...
    18. Demyanenko IA, Zakharova VV, Ilyinskaya OP, et al. Mitochondria-targeted antioxidant SkQ1 improves dermal wound healing in genetically diabetic mice. Oxidative medicine and cellular longevity. 2017;6:2017. https://doi.org/10.1155/2017/6408278 Go to original source... Go to PubMed...
    19. Ren J, Yang M, Xu F, et al. Acceleration of wound healing activity with syringic acid in streptozotocin induced diabetic rats. Life sciences. 2019;233:116728. https://doi.org/10.1016/j.lfs.2019.116728 Go to original source... Go to PubMed...
    20. Goumans MJ, Liu Z, Ten Dijke P. TGF-β signaling in vascular biology and dysfunction. Cell research. 2009;19(1):116-127. https://doi.org/10.1038/cr.2008.326 Go to original source... Go to PubMed...
    21. Wang X, Ma W, Han S, et al. TGF-β participates choroid neovascularization through Smad2/3-VEGF/TNF-α signaling in mice with Laser-induced wet age-related macular degeneration. Scientific Reports. 2017;7(1):1-3. https://doi.org/10.1038/s41598-017-10124-4 Go to original source... Go to PubMed...
    22. Meyer M. Processing of collagen based biomaterials and the resulting materials properties. Biomedical engineering online. 2019;18(1):1-74. https://doi.org/10.1186/s12938-019-0647-0 Go to original source... Go to PubMed...
    23. Kim KK, Sheppard D, Chapman HA. TGF-β1 signaling and tissue fibrosis. Cold Spring Harbor perspectives in biology. 2018;10(4):a022293. Go to original source... Go to PubMed...
    24. Costantini E, Aielli L, Serra F, et al. Evaluation of Cell Migration and Cytokines Expression Changes under the Radiofrequency Electromagnetic Field on Wound Healing In Vitro Model. International Journal of Molecular Sciences. 2022;23(4):2205. https://doi.org/10.3390/ijms23042205 Go to original source... Go to PubMed...
    25. MacGregor GA. Nifedipine and hypertension: Roles of vasodilation and sodium balance. Cardiovascular drugs and therapy. 1989;3(1):295-301. https://doi.org/10.1007/BF00148474 Go to original source... Go to PubMed...

    Return to the content