MMSL 2024, 93(1):131-139 | DOI: 10.31482/mmsl.2023.012

EVALUATION OF CYTOKERATIN-19 EXPRESSION AND OXIDATIVE STRESS STATUS ON GINGIVAL WOUND HEALING OF RABBITS TREATED WITH OMEGA-3Original article

O. Kh. Mahmoud ORCID...1, J. K. Mammdoh ORCID...1, M. G. Saeed ORCID...2*
1 Department of Dental Basic Sciences, College of Dentistry, University of Mosul, Mosul, Iraq
2 Department of Pathology and Poultry Diseases, College of Veterinary Medicine, University of Mosul, Mosul, Iraq

Background: Gingiva is a delicate tissue that protects alveolar bone against external stimuli. Gingival breaks expose alveolar bone, and the fast wounds heal the betterment of the teeth frame. We aimed to compare the influence of omega-3 on the gingival healing period after trauma and the potential involvement of cytokeratin-19 protein expression.

Methods: A total of 18 rabbits were used, after trauma-induced, these rabbits were subdivided into three groups of 6 each. Group 1 is the control group that received normal saline only, the second group received omega 3 for 10 days before the trauma, while the third group received omega 3 supplements for 10 days before the gingival injury and continued receiving them for an additional 10 days afterwards. For each time point (third and seventh day of healing), 3 rabbits were sacrificed, and tissue was collected for total antioxidant capacity and malondialdehyde measurement. Tissue fixed for histopathology and immunohistochemistry analysis.

Results: Omega 3 has shown improvement in the healing process regarding the period of healing, antioxidant parameters, and intensity of cytokeratin-19 expression compared to the control group. Moreover, rabbit groups exposed to omega 3 before trauma has shown better healing response compared to those initiated therapy with trauma.

Conclusion: Omega 3 is notably beneficial when used for both prevention and treatment since it reduces oxidative stress and improves healing processes measured by increased expression of cytokeratin-19 in epithelial tissue. Omega 3 can therefore be thought of as a possible choice for the treatment of gingival wounds and gingivitis.

Keywords: CK19; Oxidative stress; Gingival wound; Omega-3; Rabbit

Received: October 18, 2022; Revised: March 1, 2023; Accepted: March 22, 2023; Prepublished online: April 11, 2023; Published: March 1, 2024  Show citation

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Mahmoud, O.K., Mammdoh, J.K., & Saeed, M.G. (2024). EVALUATION OF CYTOKERATIN-19 EXPRESSION AND OXIDATIVE STRESS STATUS ON GINGIVAL WOUND HEALING OF RABBITS TREATED WITH OMEGA-3. MMSL, 93(1), 131-139. doi: 10.31482/mmsl.2023.012
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