MMSL X:X | DOI: 10.31482/mmsl.2025.006

MODULATION OF AUTISM SYMPTOMS IN A 13-YEAR-OLD BOY BY FOOD SUPPLEMENT JUVENILCase report

Jela Hrnciarova ORCID...1,2, Anna Hanusova1,3, Tereza Horakova4, Josef Fusek ORCID...5,6, Klara Kubelkova ORCID...5*, Ales Macela ORCID...5
1 Medical Faculty, Charles University, Hradec Kralove, Czech Republic
2 Mental Health Centre, Hradec Kralove, Czech Republic
3 HANNES Ltd., Clinic of Child and Adolescent Psychiatry in Hradec Kralove, Hradec Kralove, Czech Republic
4 Special Educational Center, Hradec Kralove, Czech Republic
5 University of Defence, Military Faculty of Medicine, Hradec Kralove, Czech Republic
6 University of Pardubice, Faculty of Health Studies, Pardubice, Czech Republic

Antidepressants, antipsychotics, anti-anxiety drugs, or sleeping pills are most often used to treat autism spectrum disorders. The treatment thus focuses on the manifestations accompanying autism, such as inattention, irritability, or sleep disorders, but does not address the essence of the social deficits of autistic children. In addition, drug treatment is often poorly tolerated. Since a certain association of autism with gut microbiota dysbiosis has been demonstrated, and given the existence of the microbiota-gut-brain axis, it can be assumed that modulation of the gut microbiota could contribute to the treatment of the essence of some Autism Spectrum Disorders category manifestations. Here, we present an example of the use of the nutritional supplement Juvenil to alleviate the manifestations of Autism Spectrum Disorders in a patient with Asperger’s syndrome.

Keywords: Autism; Juvenil; Biological response modifier; Treatment; Social behavior

Received: July 7, 2025; Revised: September 8, 2025; Accepted: September 17, 2025; Prepublished online: September 25, 2025 

Download citation

References

  1. Khachadourian V, Mahjani B, Sandin S, et al. Comorbidities in autism spectrum disorder and their etiologies. Transl Psychiatry. 2023;13:71. doi: 10.1038/s41398-023-02374-w Go to original source...
  2. Muskens JB, Velders FP, Staal WG. Medical comorbidities in children and adolescents with autism spectrum disorders and attention deficit hyperactivity disorders: a systematic review. Eur Child Adolesc Psychiatry. 2017;261093-1103. doi: 10.1007/s00787-017-1020-0 Go to original source...
  3. Bougeard C, Picarel-Blanchot F, Schmid R, et al. Prevalence of Autism Spectrum Disorder and Co-morbidities in Children and Adolescents: A Systematic Literature Review. Front Psychiatry. 2021;12:744709. doi: 10.3389/fpsyt.2021.744709 Go to original source...
  4. Madra M, Ringel R, Margolis KG. Gastrointestinal Issues and Autism Spectrum Disorder. Child Adolesc Psychiatr Clin N Am. 2020;29:501-513. doi: 10.1016/j.chc.2020.02.005 Go to original source...
  5. Lane SJ, Leão MA, Spielmann V. Sleep, Sensory Integration/Processing, and Autism: A Scoping Review. Front Psychol. 2022;13:877527. doi: 10.3389/fpsyg.2022.877527 Go to original source...
  6. Ji Q, Li S-J, Zhao J-B, et al. Genetic and neural mechanisms of sleep disorders in children with autism spectrum disorder: a review. Front Psychiatry. 2023;14:1079683. doi: 10.3389/fpsyt.2023.1079683 Go to original source...
  7. Barnard L, Muldoon K, Hasan R, et al. Profiling executive dysfunction in adults with autism and comorbid learning disability. Autism. 2008;12:125-141. doi: 10.1177/1362361307088486 Go to original source...
  8. Webb SJ, Neuhaus E, Faja S. Face perception and learning in autism spectrum disorders. Q J Exp Psychol (Hove). 2017;70:970-986. doi: 10.1080/17470218.2016.1151059 Go to original source...
  9. Welsh JP, Munson J, St John T, et al. Relationship of Impairments in Associative Learning With Intellectual Disability and Cerebellar Hypoplasia in Autistic Children. Neurology. 2023;100:e639-e650. doi: 10.1212/WNL.0000000000201496 Go to original source...
  10. Postorino V, Kerns CM, Vivanti G, et al. Anxiety Disorders and Obsessive-Compulsive Disorder in Individuals with Autism Spectrum Disorder. Curr Psychiatry Rep. 2017;19:92. doi: 10.1007/s11920-017-0846-y Go to original source...
  11. Elliott SJ, Marshall D, Morley K, et al. Behavioural and cognitive behavioural therapy for obsessive compulsive disorder (OCD) in individuals with autism spectrum disorder (ASD). Cochrane Database Syst Rev. 2021;9:CD013173. doi: 10.1002/14651858.CD013173.pub2 Go to original source...
  12. Srivastava AK, Schwartz CE. Intellectual disability and autism spectrum disorders: causal genes and molecular mechanisms. Neurosci Biobehav Rev. 2014;46 Pt 2:161-174. doi: 10.1016/j.neubiorev.2014.02.015 Go to original source...
  13. Oliver C, Licence L, Richards C. Self-injurious behaviour in people with intellectual disability and autism spectrum disorder. Curr Opin Psychiatry. 2017;30:97-101. doi: 10.1097/YCO.0000000000000313 Go to original source...
  14. Bakken TL. Behavioural equivalents of schizophrenia in people with intellectual disability and autism spectrum disorder. A selective review. Int J Dev Disabil. 2021;67:310-317. doi: 10.1080/20473869.2021.1925402 Go to original source...
  15. Cermak SA, Curtin C, Bandini LG. Food selectivity and sensory sensitivity in children with autism spectrum disorders. J Am Diet Assoc. 2010;110:238-246. doi: 10.1016/j.jada.2009.10.032 Go to original source...
  16. Malhi P, Saini S, Bharti B, et al. Sensory Processing Dysfunction and Mealtime Behavior Problems in Children With Autism. Indian Pediatr. 2021;58:842-845. Go to original source...
  17. Randell E, Wright M, Milosevic S, et al. Sensory integration therapy for children with autism and sensory processing difficulties: the SenITA RCT. Health Technol Assess. 2022;26:1-140. doi: 10.3310/TQGE0020 Go to original source...
  18. Pangrazzi L, Balasco L, Bozzi Y. Oxidative Stress and Immune System Dysfunction in Autism Spectrum Disorders. Int J Mol Sci. 2020;21:3293. doi: 10.3390/ijms21093293 Go to original source...
  19. Robinson-Agramonte M de LA, Noris García E, Fraga Guerra J, et al. Immune Dysregulation in Autism Spectrum Disorder: What Do We Know about It? Int J Mol Sci 2022; 23:3033. doi: 10.3390/ijms23063033 Go to original source...
  20. Barchel D, Stolar O, De-Haan T, et al. Oral Cannabidiol Use in Children With Autism Spectrum Disorder to Treat Related Symptoms and Co-morbidities. Front Pharmacol. 2018;9:1521. doi: 10.3389/fphar.2018.01521 Go to original source...
  21. Silva EA da, Medeiros WMB, Torro N, et al. Cannabis and cannabinoid use in autism spectrum disorder: a systematic review. Trends Psychiatry Psychother. 2022;44:e20200149. doi: 10.47626/2237-6089-2020-0149 Go to original source...
  22. Siafis S, Çiray O, Wu H, et al. Pharmacological and dietary-supplement treatments for autism spectrum disorder: a systematic review and network meta-analysis. Mol Autism. 2022;13:10. doi: 10.1186/s13229-022-00488-4 Go to original source...
  23. Fieiras C, Chen MH, Escobar Liquitay CM, et al. Risperidone and aripiprazole for autism spectrum disorder in children: an overview of systematic reviews. BMJ Evid Based Med. 2023;28:7-14. doi: 10.1136/bmjebm-2021-111804 Go to original source...
  24. Bird PD. The treatment of autism with low-dose phenytoin: a case report. J Med Case Rep. 2015;9:8. doi: 10.1186/1752-1947-9-8 Go to original source...
  25. Howes OD, Rogdaki M, Findon JL, et al. Autism spectrum disorder: Consensus guidelines on assessment, treatment and research from the British Association for Psychopharmacology. J Psychopharmacol. 2018;32:3-29. doi: 10.1177/0269881117741766 Go to original source...
  26. Maniram J, Karrim SBS, Oosthuizen F, et al. Pharmacological Management of Core Symptoms and Comorbidities of Autism Spectrum Disorder in Children and Adolescents: A Systematic Review. Neuropsychiatr Dis Treat. 2022;18:1629-1644. doi: 10.2147/NDT.S371013 Go to original source...
  27. Rzepka-Migut B, Paprocka J. Efficacy and Safety of Melatonin Treatment in Children with Autism Spectrum Disorder and Attention-Deficit/Hyperactivity Disorder-A Review of the Literature. Brain Sci. 2020;10:219. doi: 10.3390/brainsci10040219 Go to original source...
  28. Nogueira HA, de Castro CT, da Silva DCG, et al. Melatonin for sleep disorders in people with autism: Systematic review and meta-analysis. Prog Neuropsychopharmacol Biol Psychiatry. 2023;123:110695. doi: 10.1016/j.pnpbp.2022.110695 Go to original source...
  29. Adams JB, Audhya T, Geis E, et al. Comprehensive Nutritional and Dietary Intervention for Autism Spectrum Disorder-A Randomized, Controlled 12-Month Trial. Nutrients. 2018;10:369. doi: 10.3390/nu10030369 Go to original source...
  30. Adams JB, Kirby J, Audhya T, et al. Vitamin/mineral/micronutrient supplement for autism spectrum disorders: a research survey. BMC Pediatr. 2022;22:590. doi: 10.1186/s12887-022-03628-0 Go to original source...
  31. Karhu E, Zukerman R, Eshraghi RS, et al. Nutritional interventions for autism spectrum disorder. Nutr Rev. 2020;78:515-531. doi: 10.1093/nutrit/nuz092 Go to original source...
  32. Sathe N, Andrews JC, McPheeters ML, et al. Nutritional and Dietary Interventions for Autism Spectrum Disorder: A Systematic Review. Pediatrics. 2017;139:e20170346. doi: 10.1542/peds.2017-0346 Go to original source...
  33. Mayer EA, Padua D, Tillisch K. Altered brain-gut axis in autism: comorbidity or causative mechanisms? Bioessays. 2014;36:933-939. doi: 10.1002/bies.201400075 Go to original source...
  34. Ding HT, Taur Y, Walkup JT. Gut Microbiota and Autism: Key Concepts and Findings. J Autism Dev Disord. 2017;47:480-489. doi: 10.1007/s10803-016-2960-9 Go to original source...
  35. Hughes HK, Rose D, Ashwood P. The Gut Microbiota and Dysbiosis in Autism Spectrum Disorders. Curr Neurol Neurosci Rep. 2018;18:81. doi: 10.1007/s11910-018-0887-6 Go to original source...
  36. Srikantha P, Mohajeri MH. The Possible Role of the Microbiota-Gut-Brain-Axis in Autism Spectrum Disorder. Int J Mol Sci. 2019;20:2115. doi: 10.3390/ijms20092115 Go to original source...
  37. Korteniemi J, Karlsson L, Aatsinki A. Systematic review: Autism spectrum disorder and the gut microbiota. Acta Psychiatr Scand. 2023;148:242-254. doi: 10.1111/acps.13587 Go to original source...
  38. Hrnciarova J, Kubelkova K, Bostik V, et al. Modulation of Gut Microbiome and Autism Symptoms of ASD Children Supplemented with Biological Response Modifier: A Randomized, Double-Blinded, Placebo-Controlled Pilot Study. Nutrients. 2024;16:1988. doi: 10.3390/nu16131988 Go to original source...
  39. Martínez-González AE, Andreo-Martínez P. Prebiotics, probiotics and fecal microbiota transplantation in autism: A systematic review. Rev Psiquiatr Salud Ment (Engl Ed). 2020;13:150-164. doi: 10.1016/j.rpsm.2020.06.002 Go to original source...
  40. Tan Q, Orsso CE, Deehan EC, et al. Probiotics, prebiotics, synbiotics, and fecal microbiota transplantation in the treatment of behavioral symptoms of autism spectrum disorder: A systematic review. Autism Res. 2021;14:1820-1836. doi: 10.1002/aur.2560 Go to original source...
  41. Ansari F, Neshat M, Pourjafar H, et al. The role of probiotics and prebiotics in modulating of the gut-brain axis. Front Nutr. 2023;10:1173660. doi: 10.3389/fnut.2023.1173660 Go to original source...
  42. Kubelkova K, Rychlik I, Crhanova M, et al. Gut microbiota alterations by nutritional supplement Imuregen. MMSL. 2020; 89:114-125. doi: 10.31482/mmsl.2020.008 Go to original source...
  43. Bostik V, Kubelková K, Macela A. Juvenil, a natural immune booster affecting biological responses through modulation of gut microbiota composition. MMSL. 2024;93(4):364-376. doi: 10.31482/mmsl.2023.039 Go to original source...