VZL 2010, 79(1):9-14

Colorimetric Detectors Based on Acetylcholinesterase and Its Construction

Miroslav Pohanka ORCID...1,2, Vítězslav Vlček3, Jana Žďárová Karasová ORCID...2, Kamil Kuča ORCID...1,2, Jiří Cabal2
1 Univerzita obrany, Fakulta vojenského zdravotnictví, Centrum pokročilých studií, Hradec Králové
2 Univerzita obrany, Fakulta vojenského zdravotnictví, katedra toxikologie, Hradec Králové
3 Mendelova zemědělská a lesnická univerzita, Agronomická fakulta, Ústav agrochemie, půdoznalství, mikrobiologie a výživy rostlin, Brno

Kolorimetrické detektory založené na rekogniční schopnosti acetylcholinesterasy (AChE) jsou vhodným nástrojem pro rychlou a přitom vysoce senzitivní detekci nervově paralytických látek a některých pesticidů založené na inhibici AChE. V tomto přehledovém článku jsou nastíněny základní biochemické metody vhodné pro kolorimetrické stanovení aktivity AChE. Zároveň jsou popsány matrice vhodné pro konstrukci vlastních průkazníků a chemické i fyzikální metody vazby AChE na jejich povrch. Detehit a ChP-71 jsou uvedeny jako typické kolorimetrické detektory pro stanovení nervově paralytických látek.

Keywords: Kolorimetrie; Nervově paralytická látka; Acetylcholinesterasa; Průkazník; Detektor

Colorimetric detectors based on recognition capability of acetylcholinesterase (AChE) are a suitable tool for fast but sensitive detection of nerve agents and some pesticides based on AChE inhibition. In this review article, basic biochemical methods appropriate for colorimetric estimation of AChE enzymatic activity are presented. Moreover, the available matrices suitable for construction of dipsticks and chemical as well as physical immobilization protocols for AChE binding on matrice surface are described, too. Detehit and ChP-71 are indicated as typical colorimetric detectors for assay of nerve agents.

Keywords: Colorimetry; Nerve agents; Acetylcholinesterase; Dipstick; Detector

Received: November 5, 2009; Published: March 1, 2010  Show citation

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Pohanka, M., Vlček, V., Žďárová Karasová, J., Kuča, K., & Cabal, J. (2010). Colorimetric Detectors Based on Acetylcholinesterase and Its Construction. Vojenské Zdravotnické Listy, 79(1), 9-14
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