MMSL, 2015 (vol. 84), issue 4
HYDROGEN/DEUTERIUM EXCHANGE MASS SPECTROMETRY AND ITS UTILIZATIONReview article
Dominika Coufalová, Eva Michalová, Bořivoj Vojtěšek, Lenka Hernychová
MMSL 2015, 84(4):146-151 | DOI: 10.31482/mmsl.2015.018 
Hydrogen/deuterium exchange connected with mass spectrometry is increasingly applied for the interrogation of protein conformation, mapping protein dynamics, identification of protein-ligand interaction sites, and allosteric conformation changes. The dynamics of protein changes is determined with the m/z value of deuterated and non-deuterated protein or percentage of deuterium incorporation for the digested peptides. Hydrogen/deuterium exchange data are processed with selected software and finally quaternary structure of protein is visualized showing how different protein and ligand chains hook up with each other. Obtained results can help understand...
SELECTED VIRAL HEMORRHAGIC FEVERSReview article
Vanda Boštíková, Miloslav Salavec, Radek Sleha, Petr Prášil, Jan Marek, Ondřej Soukup, Kamil Kuča
MMSL 2015, 84(4):152-165 | DOI: 10.31482/mmsl.2015.019
Group of viral hemorrhagic fevers (VHF) present a range of disorders from relatively moderately serious to human life threatening entities whose mortality reaches high levels. There are classified as acute viral febrile diseases characterized by nausea, myalgia and fatigue, dominating with general alteration of vessel permeability and regulation. Symptoms of bleeding often occur, particularly in severe cases which are usually diffuse and present a symptom of general vessel injury rather than life-threatening condition.
MONITORING OF ANTHROPOMETRIC CHANGES IN THE ARMED FORCES OF THE CZECH REPUBLIC PERSONNEL DURING THE DEPLOYMENT IN AFGHANISTANOriginal article
Jan Psutka, Vladimír Pavlík, Jana Fajfrová, Miroslav Urban, Tomáš Halajčuk
MMSL 2015, 84(4):166-169 | DOI: 10.31482/mmsl.2015.020
The aim of the work is to describe the change of nutritional status and the change of physical activities in soldiers of the Armed Forces of the Czech Republic during the deployment on foreign missions in Afghanistan. As for anthropometric data, body weight, body mass index and the amount of visceral fat and muscle mass were monitored. In each monitored person the measurement was performed at least twice, the first one immediately after the beginning of the mission and the control one shortly before finishing the mission. Height was measured using a calibrated measuring device and body weight was weighed using a calibrated stand-on scale. BMI was calculated...
COMMUNICATION DISORDERS OF ACTIVE MILITARY PERSONNEL WITH TRAUMATIC BRAIN INJURY - A REVIEW STUDYReview article
Blanka Klímová, Kamil Kuča
MMSL 2015, 84(4):170-176 | DOI: 10.31482/mmsl.2015.021
Soldiers, particularly those involved in combat operations, are exposed to a continuous danger frequently resulting in various types of injuries, out of which the most common and serious is traumatic brain injury (TBI). Up to the second quarter of 2015, the Defense and Veterans Brain Injury Center has recorded 333,169 cases of active soldiers affected with TBI. TBI can be defined as an injury that results from external force to the head and causes an alternation of loss of consciousness. The key symptoms of TBI comprise physical problems, sensory impairments, behavioral changes, cognitive disorders, communication difficulties, and swallowing disorders....
EBOLA OUTBREAK IN WEST AFRICAReview article
Vanda Boštíková, Miloslav Salavec, Radek Sleha, Petr Prášil, Jan Marek, Ondřej Soukup, Kamil Kuča
MMSL 2015, 84(4):177-181 | DOI: 10.31482/mmsl.2015.022
Viral hemorrhagic fevers (VHFs) represent a collection of illnesses caused by several distinct viral families. While some types of hemorrhagic fever viruses can cause relatively mild illnesses, many of these viruses cause life-threatening diseases. In general, the term "viral hemorrhagic fever" describes a severe multisystem syndrome. Typical for this syndrome is a systemic damage of vascular system often accompanied by hemorrhages.Ebola is a type of VHF, along with Marburg, Lassa, dengue, Rift Valley, and yellow fever. VHFs are RNA viruses whose survival is dependent on animal or insect hosts. Ebola hemorrhagic fever initially makes the leap from...
HISTORY OF ORGANOPHOSPHORUS CHOLINESTERASE INHIBITORS & REACTIVATORSLetter to the editor
Georg Petroianu
MMSL 2015, 84(4):182-185 | DOI: 10.31482/mmsl.2015.023
It was with interest and pleasure that I read the contribution of Petronilho & Figueroa-Villaret in the MMSL reviewing the literature on agents for defense against chemical warfare [Petronilho & Figueroa-Villaret, 2015]. The authors briefly touch on the history of organophosphates emphasizing the pioneering contribution of Jean Louis Lassaigne, the synthesis of triethyl- phosphate (TEP) and finally the achievements of Philippe de Clermont who codeveloped the first organophosphate (OP) acetylcholinesterase inhibitor, tetraethyl pyrophosphate (TEEP). They continue by pointing out that Wilson and Ginsburg managed to reactivate OP-inhibited acetylcholinesterase...
GLANCE BACK AT THE 8th INTERNATIONAL CONFERENCE ON TULAREMIALetter to the editor
Klára Kubelková
MMSL 2015, 84(4):186-188
Tularemia is an epizootic infection caused by Francisella tularensis (F. tularensis). Tularemia is mainly a disease of wild animals that is transmitted to humans by contaminated environment or ectoparasites. Any age, sex, or race is universally susceptible to infection. Infection is incidental and is usually a result of interaction with biting or blood-sucking insects, wild animals, or their environment. F. tularensis is an etiologic agent of tularemia and, with rare exception, the only disease produced by this genus. The organism is a small, Gram-negative, pleomorphic, nonmotile, nonspore-forming coccobacillus. It is a strict aerobe...
REPORT ON THE INTERNSHIP AT QUEEN ASTRID MILITARY HOSPITAL BURNS CENTRE IN BRUSSELS, BELGIUMLetter to the editor
Adam Bajus
MMSL 2015, 84(4):189-192
A report about experiences gained during a month internship at the Queen Astrid Military Hospital Burns Centre in Brussels, Belgium, in May 2015.